Terminal Deoxynucleotidyl Transferase in the Synthesis and Modification of Nucleic Acids
Terminal Deoxynucleotidyl Transferase in the Synthesis and Modification of Nucleic Acids
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DOI：
10.1002/cbic.201800658
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发表时间：
2019-01
期刊：
影响因子：
3.2
通讯作者：
I. Sarac;M. Hollenstein
中科院分区：
文献类型：
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作者：
I. Sarac;M. Hollenstein
末端脱氧核苷酸转移酶(TDT)属于DNA聚合酶X家族。这种不寻常的聚合酶在V(D)J重组过程中催化随机核苷酸在3‘-突起上的模板无关的加成。TDT的生物学功能和内在的生化特性刺激了许多基于寡核苷酸的工具和方法的发展，特别是如果与修饰的核苷三磷酸结合的话。在这里，我们总结了不同的应用源于TDT掺入修饰的核苷酸。文中还讨论了该聚合酶的结构、作用机理和生化性质。
The terminal deoxynucleotidyl transferase (TdT) belongs to the X family of DNA polymerases. This unusual polymerase catalyzes the template‐independent addition of random nucleotides on 3′‐overhangs during V(D)J recombination. The biological function and intrinsic biochemical properties of the TdT have spurred the development of numerous oligonucleotide‐based tools and methods, especially if combined with modified nucleoside triphosphates. Herein, we summarize the different applications stemming from the incorporation of modified nucleotides by the TdT. The structural, mechanistic, and biochemical properties of this polymerase are also discussed.