Saccharonzyces boulardii produces a soluble anti-inflammatory factor that inhibits NF-κB-mediated IL-8 gene expression
Saccharonzyces boulardii produces a soluble anti-inflammatory factor that inhibits NF-κB-mediated IL-8 gene expression
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DOI：
10.1016/j.bbrc.2006.02.080
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发表时间：
2006-04-28
影响因子：
3.1
通讯作者：
Kelly, CP
中科院分区：
文献类型：
--
作者：
Sougioultzis, S;Simeonidis, S;Kelly, CP
布拉酵母菌（S6）是一种非致病性酵母，可改善由多种肠道病原体引起的肠道损伤和炎症。我们推测Sb可能通过调节宿主细胞信号传导和促炎基因表达来发挥其益生菌效应。人HT-29结肠细胞和THP-1单核细胞在Sb培养上清液（SbS）存在或不存在的情况下用IL-1 β、TNF α或LPS刺激。ELISA法检测IL-8蛋白表达，RT-PCR法检测IL-8 mRNA表达。通过Western印迹法研究了SbS对I κ B α降解的影响，并通过EMSA研究了SbS对NF-κ B-DNA结合的影响。通过用NF-κ B应答荧光素酶报告基因瞬时转染THP-1细胞来评估NF-κ B调节的基因表达。SbS抑制IL-1 β或TNF α刺激的HT-29细胞中IL-8蛋白的产生（分别为75%和85%; P < 0.001），并阻止IL-1 β诱导的IL-8 mRNA的上调。在IL-1 β或LPS刺激的THP-1细胞中，SBS还抑制IL-8的产生，阻止I κ B α降解，并降低NF-κ B-DNA结合和NF-κ B报告基因上调。纯化和表征研究表明，S。布拉氏菌抗炎因子（SAIF）小（< 1 kDa）、热稳定且水溶性。益生菌酵母布拉酵母通过产生低分子量可溶性因子发挥抗炎作用，所述低分子量可溶性因子阻断肠上皮细胞和单核细胞中NF-κ B活化和NF-κ B介导的IL-8基因表达。SAIF可以至少部分地介导布拉酵母在感染性和非感染性人类肠道疾病中的有益作用。(c)2006年由Elsevier Inc.出版
Saccharomyces boulardii (S6) is a non-pathogenic yeast that ameliorates intestinal injury and inflammation caused by a wide variety of enteric pathogens. We hypothesized that Sb may exert its probiotic effects by modulation of host cell signaling and pro-inflammatory gene expression. Human HT-29 colonocytes and THP-1 rnonocytes were stimulated with IL-1 beta, TNF alpha or LPS in the presence or absence of Sb culture supernatant (SbS). IL-8 protein and mRNA levels were measured by ELISA and RT-PCR, respectively. The effect of SbS on I kappa B alpha degradation was studied by Western blotting and on NF-kappa B-DNA binding by EMSA. NF-kappa B-regulated gene expression was evaluated by transient transfection of THP-1 cells with a NF-kappa B-responsive luciferase reporter gene. SbS inhibited IL-8 protein production in IL-1 beta or TNF alpha stimulated HT-29 cells (by 75% and 85%, respectively; P < 0.001) and prevented IL-1 beta-induced up-regulation of IL-8 mRNA. Sbs also inhibited IL-8 production, prevented I kappa B alpha degradation, and reduced both NF-kappa B-DNA binding and NF-kappa B reporter gene up-regulation in IL-1 beta or LPS-stimulated THP-1 Cells. Purification and characterization studies indicate that the S. boulardii anti-inflammatory factor (SAIF) is small (< 1 kDa), heat stable, and water soluble. The probiotic yeast Saccharomyces boulardii exerts an anti-inflammatory effect by producing a low molecular weight soluble factor that blocks NF-kappa B activation and NF-kappa B-mediated IL-8 gene expression in intestinal epithelial cells and monocytes. SAIF may mediate, at least in part, the beneficial effects of Saccharomyces boulardii in infectious and non-infectious human intestinal disease. (c) 2006 Published by Elsevier Inc.