Serum Hydroxyl Radical Scavenging Capacity as Quantified with Iron-Free Hydroxyl Radical Source
Serum Hydroxyl Radical Scavenging Capacity as Quantified with Iron-Free Hydroxyl Radical Source
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DOI：
10.3164/jcbn.08-265
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发表时间：
2009-09-01
影响因子：
2.4
通讯作者：
Kotake, Yashige
中科院分区：
文献类型：
--
作者：
Endo, Nobuyuki;Oowada, Shigeru;Kotake, Yashige
我们开发了一种简单的基于ESR自旋捕获的方法来测定羟基（OR）自由基清除能力，使用不含铁的OR自由基源。与广泛使用的芬顿反应不同，我们采用了短时间（通常为5秒）的原位紫外光解稀过氧化氢水溶液，以产生可重复量的OR自由基。ESR自旋捕获用于量化OR自由基；由于标本的清除活性而导致的OR自由基水平的下降被转化为OR自由基清除能力（速率）。在纯抗氧化剂中证实了该方法的有效性，与前人的数据吻合较好。在这项工作的后半段，新方法被应用于慢性肾功能衰竭（CRF）患者的血清。我们首次发现血液透析后，CRF血清的OR自由基清除能力恢复到健康对照水平。该方法简单快速，低浓度过氧化氢是唯一添加到系统中的化学物质，可以消除涉及铁的芬顿反应的复杂性或使用脉冲辐射分解系统。
We have developed a simple ESR spin trapping based method for hydroxyl (OR) radical scavenging-capacity determination, using iron-free OR radical source. Instead of the widely used Fenton reaction, a short (typically 5 seconds) in situ UV-photolysis of a dilute hydrogen peroxide aqueous solution was employed to generate reproducible amounts of OR radicals. ESR spin trapping was applied to quantify OR radicals; the decrease in the OR radical level due to the specimen's scavenging activity was converted into the OR radical scavenging capacity (rate). The validity of the method was confirmed in pure antioxidants, and the agreement with the previous data was satisfactory. In the second half of this work, the new method was applied to the sera of chronic renal failure (CRF) patients. We show for the first time that after hemodialysis, OR radical scavenging capacity of the CRF serum was restored to the level of healthy control. This method is simple and rapid, and the low concentration hydrogen peroxide is the only chemical added to the system, that could eliminate the complexity of iron-involved Fenton reactions or the use of the pulse-radiolysis system.