INDUCTION OF A TRANSIENT ENHANCEMENT OF LOW LEVEL CHEMILUMINESCENCE IN INTACT LEAVES BY ANAEROBIC TREATMENT
INDUCTION OF A TRANSIENT ENHANCEMENT OF LOW LEVEL CHEMILUMINESCENCE IN INTACT LEAVES BY ANAEROBIC TREATMENT
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通过厌氧处理诱导完整叶子中低水平化学发光的瞬时增强
DOI：
10.1111/j.1751-1097.1992.tb02159.x
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发表时间：
1992
影响因子：
3.3
通讯作者：
Humio Inaba
中科院分区：
文献类型：
--
作者：
P. Roschger;B. Devaraj;R. Q. Scott;Humio Inaba
当环境空气被厌氧大气取代时，适应黑暗的完整叶片的内在低水平化学发光表现出一种瞬时的光发射增强。在缺氧后阶段，重新建立好氧条件会引起复杂的光发射动力学行为。叶片内的代谢变化被认为是造成这种现象的原因，在这种情况下，叶片的完整性是至关重要的。叶片匀浆表现出几乎不依赖氧的化学发光。相比之下，紫外光损伤叶片和叶绿体制剂的低水平化学发光被缺氧抑制。对完整叶片的超弱光发射光谱分析表明，在好氧和厌氧情况下，超弱光的来源是相同的。从叶片中获得的延迟荧光光谱的相似性表明叶绿素是发光物种。
Intrinsic low level chemiluminescence of dark adapted intact leaves exhibits a transient enhancement of light emission when ambient air is replaced with an anaerobic atmosphere. Re establishing aerobic conditions gives rise to a complex kinetic behavior of the light emission in the post‐anoxic phase. Metabolic changes within the leaf are suggested to be responsible for this phenom enon, where intactness of the leaf is found to be of crucial importance. Leaf homogenate showed nearly oxygen independent chemiluminescence. In contrast, low level chemiluminescence of UV‐light damaged leaves and of chloroplast preparations was inhibited by oxygen depletion. Spectral analyses of the ultraweak light emission from the intact leaf indicates that in both aerobic and anaerobic cases, the source of the ultraweak light is the same. The similarity to the spectrum of delayed fluorescence also obtained from the leaf suggests that chlorophyll is the light emitting species.