Conversion of dehydroepiandrosterone to downstream steroid hormones in macrophages
Conversion of dehydroepiandrosterone to downstream steroid hormones in macrophages
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DOI：
10.1677/joe.0.1640161
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发表时间：
2000-02-01
影响因子：
4
通讯作者：
Straub, RH
中科院分区：
文献类型：
--
作者：
Schmidt, M;Kreutz, M;Straub, RH
脱氢表雄酮 (DHEA) 是一种普遍存在的肾上腺激素，具有免疫调节作用，例如抑制单核因子的产生。 DHEA 本身或下游类固醇是否是靶细胞中的免疫调节效应激素尚不清楚。在本研究中，我们研究了目标巨噬细胞中 DHEA 向下游类固醇激素的转化。在用放射性标记 DHEA 培养 1 天内，单核细胞来源的巨噬细胞将 DHEA 转化为大量的 Delta 5-衍生物，例如 160H-DHEA、3β,17β-雄烯二醇 (A'diol) 和 3β,16α,17β-雄烯三醇（三醇）。然而，Delta 4-类固醇（雄烯二酮（A'dione）、睾酮（T）和16OH-T）和雌激素（雌酮、雌二醇和雌三醇）的产生相对较低。用放射性标记的 DHEA 进一步培养巨噬细胞 5 天，结果显着 (P
Dehydroepiandrosterone (DHEA) is a ubiquitous adrenal hormone with immunomodulatory effects such as inhibition of the production of monokines. Whether DHEA itself or the downstream steroids are the immunomodulatory effector hormones in target cells is not known. In this study, we investigated the conversion of DHEA to downstream steroid hormones in target macrophages.Within 1 day of culture with radiolabeled DHEA, monocyte-derived macrophages converted DHEA to significant amounts of Delta 5-derivatives such as 160H-DHEA, 3 beta,17 beta-androstenediol (A'diol), and 3 beta,16 alpha,17 beta-androstenetriol (A'triol). However, the production of Delta 4-steroids (androstenedione (A'dione), testosterone (T), and 16OH-T) and estrogens (estrone, estradiol, and estriol) was relatively low. Further cultivation of macrophages for 5 days with radiolabeled DHEA resulted in a significant (P