The influence of diet on faecal DNA amplification and sex identification in brown bears (Ursus arctos)
The influence of diet on faecal DNA amplification and sex identification in brown bears (Ursus arctos)
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DOI：
10.1046/j.1365-294x.2003.01863.x
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发表时间：
2003-08-01
影响因子：
4.9
通讯作者：
Kendall, KC
中科院分区：
文献类型：
--
作者：
Murphy, MA;Waits, LP;Kendall, KC
为了评估饮食对粪便DNA扩增的影响，将11只圈养棕熊（Ursus arctos）置于六种限制性饮食中：草（三叶草属，Haplopappus hirtus和Poa pratensis）、苜蓿（Lupinus spp.）、胡萝卜（胡萝卜属），白尾鹿（Odocoileus virginianus）、蓝莓（Vaccinium spp.）和鲑鱼（萨尔莫属）。从每种限制饮食的50个粪便样品中提取DNA，并尝试扩增棕熊DNA的线粒体DNA（mtDNA）位点和核DNA（nDNA）位点。对于mtDNA，在不同饮食中没有观察到扩增成功率的显著差异。对于nDNA，鲑鱼饮食提取物的扩增成功率显著低于所有其他饮食提取物（P < 0.001）。为了评估雌性食肉动物食用雄性哺乳动物猎物时粪便DNA性别鉴定的准确性，雌性熊喂食雄性白尾鹿。10份提取物中有4份扩增，由于X和Y染色体片段扩增，所有提取物均被错误评分为雄性。本研究中强调的潜在偏见对使用粪便DNA进行个体和性别识别的研究人员具有广泛的影响，并且应该在其他物种中进行评估。
To evaluate the influence of diet on faecal DNA amplification, 11 captive brown bears (Ursus arctos ) were placed on six restricted diets: grass (Trifolium spp., Haplopappus hirtus and Poa pratensis ), alfalfa (Lupinus spp.), carrots (Daucus spp.), white-tailed deer (Odocoileus virginianus ), blueberries (Vaccinium spp.) and salmon (Salmo spp.). DNA was extracted from 50 faecal samples of each restricted diet, and amplification of brown bear DNA was attempted for a mitochondrial DNA (mtDNA) locus and nuclear DNA (nDNA) locus. For mtDNA, no significant differences were observed in amplification success rates across diets. For nDNA, amplification success rates for salmon diet extracts were significantly lower than all other diet extracts (P < 0.001). To evaluate the accuracy of faecal DNA sex identification when female carnivores consume male mammalian prey, female bears were fed male white-tailed deer. Four of 10 extracts amplified, and all extracts were incorrectly scored as male due to amplification of X and Y-chromosome fragments. The potential biases highlighted in this study have broad implications for researchers using faecal DNA for individual and sex identification, and should be evaluated in other species.