Probiotics modulate gut microbiota and health status in Japanese cedar pollinosis patients during the pollen season
Probiotics modulate gut microbiota and health status in Japanese cedar pollinosis patients during the pollen season
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DOI：
10.1007/s00394-016-1264-3
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发表时间：
2017-10-01
影响因子：
5
通讯作者：
Salminen, Seppo
中科院分区：
文献类型：
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作者：
Harata, Gaku;Kumar, Himanshu;Salminen, Seppo
日本雪松花粉症（JCP）是日本一个具有挑战性的公共卫生问题。肠道微生物群的改变与几种疾病有关，包括过敏性疾病。然而，只有少数研究关注JCP，益生菌作用的潜在机制仍不清楚。此外，本研究首次观察了JCP患者肠道菌群与血脂之间的相关性。方法收集14例JCP患者在LGG-TMC 0356发酵乳治疗前后（n = 14）和11例LGG-TMC 0356发酵乳治疗后（n = 11）的粪便样本。结果基于16 S rRNA的微生物操作分类单元聚类结果显示，LGG-TMC 0356发酵乳显著改变了肠道菌群，共检测到8个优势菌属。在JCP季节期间，与基线相比，研究结束时受试者的拟杆菌/厚壁菌比率显著降低。拟杆菌与LDL-和HDL-胆固醇水平呈正相关，而厚壁菌门与总胆固醇、LDL-和HDL-胆固醇水平呈负相关。结论通过补充含有研究益生菌的发酵乳改变肠道菌群可能是预防JCP的一个前瞻性目标，对血脂水平具有有益影响。
Background Japanese cedar pollinosis (JCP) is a challenging public health problem in Japan. Altered gut microbiota is associated with several diseases, including allergic diseases. However, only a few studies have focused on JCP and the underlying mechanisms for probiotic effects remain unclear. In addition, this study is the first observation of the correlation between the gut microbiota and blood lipid in JCP.Methods Faecal samples from JCP subjects were collected before and after treatment with (n = 14) and without (n = 11) LGG-TMC0356-fermented milk for 10 weeks. Gut microbiota composition was characterized from faecal DNA using sequencing of 16S rRNA genes.Results 16S rRNA-based operational taxonomic unit clustering of the microbiota revealed that LGG-TMC0356-fermented milk significantly altered gut microbiota after 10 weeks of milk consumption, and eight dominant genera of microbes were detected. During the JCP season, the Bacteroidetes/Firmicutes ratio, when compared to baseline, was significantly decreased in subjects at end of the study. Bacteroidetes showed positive correlation with LDL- and HDL-cholesterol levels, whereas Firmicutes showed negative correlation with total cholesterol, LDL- and HDL- cholesterol.Conclusions The altered gut microbiota through supplementation of fermented milk containing the study probiotics may be a prospective target for protection against JCP, with beneficial effects on blood lipid levels.