Spectral photoacoustic imaging to estimate in vivo placental oxygenation during preeclampsia
Spectral photoacoustic imaging to estimate in vivo placental oxygenation during preeclampsia
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DOI：
10.1038/s41598-018-37310-2
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发表时间：
2019-01-24
影响因子：
4.6
通讯作者：
Bayer, Carolyn L.
中科院分区：
文献类型：
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作者：
Lawrence, Dylan J.;Escott, Megan E.;Bayer, Carolyn L.
子痫前期是一种妊娠相关的高血压疾病，占全球每年孕产妇死亡的14%。子痫前期——产妇高血压和蛋白尿——是由子宫胎盘灌注减少导致的胎盘缺血引起的。在这里，我们使用光谱光声成像评估子痫前期胎盘氧合的纵向变化。获取正常妊娠大鼠（NP）和子痫前期子宫灌注压降低大鼠（RUPP）妊娠第14、16和18天（妊娠中后期）胎盘的光谱光声图像（每组n = 10）。RUPP手术模型实施2天后，胎盘氧饱和度较NP降低12%。蛋白尿由GD18成像前24小时尿液收集确定。成像后在GD18上测量血压。通过缺氧诱导因子(HIF)-1 α的组织学染色证实了RUPP胎盘缺氧，HIF -1 α是一种响应局部氧水平的细胞转录调节因子。采用体内纵向成像方法，我们确定子痫前期子宫灌注压降低大鼠模型胎盘缺氧，并且这种缺氧持续到妊娠后期。未来的工作将利用这些方法来评估新疗法对胎盘缺血和子痫前期进展的影响。
Preeclampsia is a pregnancy-related hypertensive disorder accounting for 14% of global maternal deaths annually. Preeclampsia-maternal hypertension and proteinuria-is promoted by placental ischemia resulting from reduced uteroplacental perfusion. Here, we assess longitudinal changes in placental oxygenation during preeclampsia using spectral photoacoustic imaging. Spectral photoacoustic images were acquired of the placenta of normal pregnant (NP) and preeclamptic reduced uterine perfusion pressure (RUPP) Sprague Dawley rats on gestational days (GD) 14, 16, and 18, corresponding to mid- to late gestation (n = 10 per cohort). Two days after implementation of the RUPP surgical model, placental oxygen saturation decreased 12% in comparison with NP. Proteinuria was determined from a 24-hour urine collection prior to imaging on GD18. Blood pressure measurements were obtained on GD18 after imaging. Placental hypoxia in the RUPP was confirmed with histological staining for hypoxia-inducible factor (HIF)-1 alpha, a cellular transcription regulator which responds to local oxygen levels. Using in vivo, longitudinal imaging methods we determined that the placenta in the reduced uterine perfusion pressure rat model of preeclampsia is hypoxic, and that this hypoxia is maintained through late gestation. Future work will utilize these methods to assess the impact of novel therapeutics on placental ischemia and the progression of preeclampsia.