Nitrate reductase, a nitric oxide-producing enzyme: induction by pathogen signals
Nitrate reductase, a nitric oxide-producing enzyme: induction by pathogen signals
复制标题
DOI：
10.1007/s10327-003-0039-x
复制
发表时间：
2003-08
影响因子：
1.2
通讯作者：
A. Yamamoto;S. Katou;H. Yoshioka;N. Doke;K. Kawakita
中科院分区：
文献类型：
--
作者：
A. Yamamoto;S. Katou;H. Yoshioka;N. Doke;K. Kawakita
一氧化氮（NO）被认为是植物细胞防御信号的效应器。硝酸还原酶（NR）是植物体内产生no的酶之一。在这里，我们报道了马铃薯晚疫病的真菌病原体疫霉侵染马铃薯块茎后，在不相容而非不相容的相互作用中引起了thenr转录的短暂增加。真菌诱导子菌丝壁成分（HWC）处理马铃薯块茎在转录和蛋白水平上诱导nrgene。hwc处理块茎的可溶性蛋白表现出增强的亚硝酸盐依赖性NO生成。蛋白激酶和细胞外Ca2+对hwc诱导的thenr转录物积累的抑制实验表明，一种钙无关的蛋白激酶参与了theNRgene的调控。此外，我们从马铃薯块茎cDNA文库中分离到编码NR的cDNA克隆；两个同基因分别被命名为stnr5和stnr6。利用基因特异性引物进行逆转录-聚合酶链反应分析表明，HWC在马铃薯块茎中诱导了stnr5和stnr6的转录本。这是首次报道病原体信号诱导theNRgene的表达。
Nitric oxide (NO) is believed to act as an effector for defense signaling in plant cells. Nitrate reductase (NR) is one of the NO-producing enzymes in plants. Here, we report that infection ofPhytophthora infestans, the fungal pathogen of potato late blight, into potato tubers caused a transient increase in theNRtranscript in an incompatible, but not a compatible, interaction. Treatment of potato tubers with the fungal elicitor hyphal wall components (HWC) from the fungus inducedNRgene at the transcriptional and protein level. Soluble proteins from HWC-treated tubers exhibited enhanced nitrite-dependent NO production. The inhibitor experiments for protein kinase and extracellular Ca2+on the HWC-induced accumulation of theNRtranscript suggested involvement of a calcium-independent protein kinase in regulation of theNRgene. Additionally, we isolated cDNA clones encoding NR from a potato tuber cDNA library; two isogenes were designatedStNR5andStNR6, respectively. Reverse transcription-polymerase chain reaction analyses using gene-specific primers showed that transcripts for bothStNR5andStNR6were induced by HWC in potato tubers. This is the first report that pathogen signals induce expression of theNRgene.