Structure and expression of the triose phosphate isomerase (Tpi) gene of Drosophila melanogaster.
Structure and expression of the triose phosphate isomerase (Tpi) gene of Drosophila melanogaster.
复制标题
果蝇磷酸丙糖异构酶（Tpi）基因的结构和表达。
DOI：
10.1007/bf00290672
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Sullivan,DT
中科院分区：
文献类型：
--
作者：
Shaw-Lee,RL;Lissemore,JL;Sullivan,DT
We report the isolation of the genomic sequence that encodes the enzyme triose phosphate isomerase ofDrosophila melanogaster. There is a single copy of theTpisequence in the genome of Drosophila, as judged by Southern blots andin situhybridization to salivary gland chromosomes. The sequence of 3414 nucleotides from theTpiregion was determined. The gene has an intron in the 5′ untranslated region of the transcript and a second intron in the coding region at an evolutionarily conserved position. Transcripts initiate at a single site which does not have a TATA box in the usual position. Northern blot analysis of RNA prepared from different developmental stages revealed thatTpimRNA is present in substantial amounts in oocytes, declines in abundance in early embryos, and begins to increase during mid-embryogenesis. Transcript abundance follows a pattern typical of enzymes involved in intermediate metabolism. A peak is found during third instar followed by a decline during pupal stages and then a second rise near the time of eclosion.
