Activatable optical imaging with a silica-rhodamine based near infrared (SiR700) fluorophore: a comparison with cyanine based dyes.
Activatable optical imaging with a silica-rhodamine based near infrared (SiR700) fluorophore: a comparison with cyanine based dyes.
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DOI：
10.1021/bc2003617
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发表时间：
2011-12-21
影响因子：
4.7
通讯作者：
Kobayashi, Hisataka
中科院分区：
文献类型：
--
作者：
McCann, Thomas E.;Kosaka, Nobuyuki;Koide, Yuichiro;Mitsunaga, Makoto;Choyke, Peter L.;Nagano, Tetsuo;Urano, Yasuteru;Kobayashi, Hisataka
光学成像正在成为可视化肿瘤的重要工具。然而，可用的荧光团有许多潜在的选择。在可见光范围内发射的光学成像探针可以以高量子产率对浅表肿瘤进行成像，但是，如果需要更深的成像，则需要近红外 (NIR) 荧光团。大多数市售近红外荧光团都是花青基的，容易发生非特异性结合和相对有限的光稳定性。含二氧化硅的罗丹明 (SiR) 荧光团代表了一类新型 NIR 荧光团，它允许通过 H-二聚体形成进行光活化，并表现出改善的光稳定性。这使得在更长的时间内实现更高的肿瘤与背景比（TBR）。在这里，我们在卵巢癌转移的小鼠肿瘤模型中将与 SiR700 缀合的亲和素 (Av-SiR700) 与基于花青染料的类似化合物（Av-Cy5.5 和 Av-Alexa Fluor 680）进行了比较。我们发现 Av-SiR700 探针表现出卓越的猝灭能力，能够在与靶细胞结合内化后激活。因此，与 Av-Cy5.5 相比，Av-SiR700 具有更高的 TBR，与 Av-Alexa Fluor 680 相比具有更好的生物稳定性。
Optical imaging is emerging as an important tool to visualize tumors. However, there are many potential choices among the available fluorophores. Optical imaging probes that emit in the visible range can image superficial tumors with high quantum yields, however, if deeper imaging is needed then near infrared (NIR) fluorophores are necessary. Most commercially available NIR fluorophores are cyanine based and are prone to non-specific binding and relatively limited photostability. Silica-containing rhodamine (SiR) fluorophores represent a new class of NIR fluorophores, which permit photoactivation via H-dimer formation as well as demonstrate improved photostability. This permits higher tumor-to-background ratios (TBRs) to be achieved over longer periods of time. Here, we compared an avidin conjugated with SiR700 (Av-SiR700) to similar compounds based on cyanine dyes (Av-Cy5.5 and Av-Alexa Fluor 680) in a mouse tumor model of ovarian cancer metastasis. We found that the Av-SiR700 probe demonstrated superior quenching enabling activation after binding-internalization to the target cell. As a result, Av-SiR700 had higher TBRs compared to Av-Cy5.5, and better biostability compared to Av-Alexa Fluor 680.
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影响因子：
5.7
作者：
Ogawa M;Regino CA;Choyke PL;Kobayashi H
通讯作者：
Kobayashi H
影响因子：
2.8
作者：
ARBELOA, IL;OJEDA, PR
通讯作者：
OJEDA, PR
影响因子：
62.1
作者：
Kobayashi, Hisataka;Ogawa, Mikako;Alford, Raphael;Choyke, Peter L.;Urano, Yasuteru
通讯作者：
Urano, Yasuteru
影响因子：
--
作者：
Ogawa M;Kosaka N;Regino CA;Mitsunaga M;Choyke PL;Kobayashi H
通讯作者：
Kobayashi H
影响因子：
11.5
作者：
Hama, Yukihiro;Urano, Yasuteru;Kobayashi, Hisataka
通讯作者：
Kobayashi, Hisataka