Taiman acts as a coactivator of Yorkie in the Hippo pathway to promote tissue growth and intestinal regeneration.
Taiman acts as a coactivator of Yorkie in the Hippo pathway to promote tissue growth and intestinal regeneration.
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Taiman 在 Hippo 通路中充当 Yorkie 的共激活剂，促进组织生长和肠道再生
DOI：
10.1038/celldisc.2016.6
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发表时间：
2016
期刊：
影响因子：
33.5
通讯作者：
Zhang L
中科院分区：
文献类型：
--
作者：
Wang C;Yin MX;Wu W;Dong L;Wang S;Lu Y;Xu J;Wu W;Li S;Zhao Y;Zhang L
Hippo 信号通路通过控制细胞生长、增殖和凋亡来调节组织生长和器官大小。在这些过程中，共激活因子 Yorkie 与转录因子 Scalloped 合作，介导 Hippo 通路调节的细胞功能。在这里，我们证明 Taiman 促进了 Yorkie 的活动。首先，Taiman 过度表达上调 Hippo 通路响应基因并诱导组织过度生长。其次，tai 的缺失会下调 Hippo 途径靶基因的表达，并减少器官大小以及由 Yorkie 过度表达引起的组织过度生长。此外，我们提供的证据表明 Taiman 与 Yorkie 结合并促进 Yorkie-Scalloped 的活性，从而激活多个 Hippo 通路靶基因的转录。此外，我们发现Taiman的C端对于Taiman在Hippo信号传导中的功能是不可或缺的。最后，我们证明 Taiman 也是肠道干细胞增殖所必需的。我们的研究结果表明泰曼是约克夏的重要共同激活剂。
The Hippo signaling pathway regulates tissue growth and organ size through controlling cell growth, proliferation and apoptosis. During these processes, the coactivator Yorkie partners with the transcription factor Scalloped to mediate Hippo pathway-regulated cellular functions. Here, we demonstrate that Taiman facilitates the activity of Yorkie. First, Taiman overexpression upregulates Hippo pathway-responsive genes and induces tissue overgrowth. Second, the loss of tai downregulates the expression of Hippo pathway target genes and reduces organ size as well as tissue overgrowth caused by Yorkie overexpression. Furthermore, we provide evidence that Taiman binds to Yorkie and facilitates the activity of Yorkie-Scalloped to activate the transcription of several Hippo pathway target genes. Moreover, we found that the C-terminus of Taiman is indispensable for the function of Taiman in Hippo signaling. Finally, we demonstrate that Taiman is also required in intestinal stem cell proliferation. Our findings suggest Taiman is an essential coactivator of Yorkie.