Combined Markers to Assess Meat Intake-Human Metabolomic Studies of Discovery and Validation
Combined Markers to Assess Meat Intake-Human Metabolomic Studies of Discovery and Validation
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DOI：
10.1002/mnfr.201900106
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发表时间：
2019-09-01
影响因子：
5.2
通讯作者：
Dragsted, Lars O.
中科院分区：
文献类型：
--
作者：
Cuparencu, Catalina;Rinnan, Asmund;Dragsted, Lars O.
红肉的生物标志物可以澄清肉类摄入量与健康之间的关系。本文探讨了不同血红素铁含量的三种类型的肉类的摄入量的生物标志物的发现。红肉和一般肉类的候选生物标志物将根据定义的验证标准进行进一步评估。方法和结果在一项随机交叉膳食研究中，健康志愿者食用随机序列的四种测试餐：鸡肉，猪肉，牛肉，和由鸡蛋白色和豌豆制成的对照。收集空腹和餐后尿样以覆盖48小时，并通过非靶向LC-ESI-qTOF-MS代谢组学进行分析。通过单变量和多变量分析探索膳食挑战后的特征。九个红色，四个白色，和八个一般的肉生物标志物被选为推定的生物标志物，来源于胶原蛋白降解，风味化合物，和氨基酸代谢。血红素相关的代谢产物被对照餐的叶绿素含量掩盖。候选生物标志物在独立的膳食研究中得到确认，并验证了可扩展性、稳健性、时间响应和预测性能。生物标志物的组合在预测肉类摄入量方面比单一标志物更有效。结论提出了部分验证的生物标志物的新组合，以评估陆地肉类摄入量，从而有助于解开肉类消费对人类健康的影响。
Scope Biomarkers of red meat may clarify the relationship between meat intake and health. This paper explores the discovery of biomarkers of intake for three types of meat with varying heme iron content. Candidate biomarkers for red and general meat are further evaluated based on defined validation criteria. Methods and results In a randomized cross-over meal study, healthy volunteers consume a randomized sequence of four test meals: chicken, pork, beef, and a control made of egg white and pea. Fasting and postprandial urine samples are collected to cover 48 h and profiled by untargeted LC-ESI-qTOF-MS metabolomics. The profiles following the meal challenges are explored by univariate and multivariate analyses. Nine red, four white, and eight general meat biomarkers are selected as putative biomarkers, originating from collagen degradation, flavour compounds, and amino acid metabolism. Heme-related metabolites are masked by the chlorophyll content of the control meal. The candidate biomarkers are confirmed in an independent meal study and validated for plausibility, robustness, time-response, and prediction performance. Combinations of biomarkers are more efficient than single markers in predicting meat intake. Conclusion New combinations of partially validated biomarkers are proposed to assess terrestrial meat intake and thus help disentangle the effects of meat consumption on human health.