Immunocompetent cells from the lower respiratory tract of normal human lungs.
Immunocompetent cells from the lower respiratory tract of normal human lungs.
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来自正常人肺下呼吸道的免疫活性细胞。
DOI：
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发表时间：
1975
影响因子：
15.9
通讯作者：
D T Rowlands
中科院分区：
文献类型：
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作者：
R. P. Daniele;M D Altose;D T Rowlands
正常人肺支气管肺泡气隙中淋巴细胞亚群与外周血中淋巴细胞亚群进行了比较。通过补体受体（EAC受体）和表面免疫球蛋白鉴定骨髓来源的（囊等价物）细胞（B细胞）。胸腺来源的淋巴细胞（T细胞）通过它们对有丝分裂原的增殖反应和E玫瑰花结技术鉴定。用纤维支气管镜对8例不吸烟的健康志愿者进行肺段灌洗，回收肺内细胞。平均而言，巨噬细胞占抽吸物中细胞的78%，淋巴细胞占17%。表面免疫球蛋白和补体受体检测到的B细胞分别相当于肺淋巴细胞的22%和15%。肺B细胞在重链免疫球蛋白类别中的分布显示IgM和IgG是主要类别，平均值分别为14.5%和9.3%;伊加的相应值为5%。在同一受试者和其他对照受试者中，观察到纯化的外周血淋巴细胞的频率顺序相当（IgM大于IgG大于伊加）。通过E玫瑰花结法，T细胞占可识别肺淋巴细胞的大多数（47%）。肺T细胞的存在也证实了他们的增殖反应的有丝分裂原（植物血凝素和刀豆球蛋白A），但反应是小于相同数量的外周血淋巴细胞从相同的主题。肺淋巴细胞的B/T细胞比与相同受试者外周血淋巴细胞的结果相当，但较高比例的肺淋巴细胞不能被鉴定为T或B细胞。据推测，肺淋巴细胞参与肺的局部免疫防御。
Subpopulations of lymphocytes in the broncho-alveolar air spaces of normal human lungs were compared with those in peripheral blood. Bone marrow-derived (bursal-equivalent) cells (B cells) were identified by complement receptors (EAC rosettes) and by surface immunoglobulin. Thymus-derived lymphocytes (T cells) were identified by their proliferative response to mitogens and the E rosette technique. Cells in lung air spaces were recovered from eight healthy nonsmoking volunteers by segmental lavage with the flexible bronchofiberscope. On the average, macrophages constituted 78% and lymphocytes 17% of the cells in the aspirates. B cells detected by surface immunoglobulin and complement receptors equaled 22% and 15% of lung lymphocytes, respectively. The distribution of lung B cells into heavy chain immunoglobulin classes revealed IgM and IgG to be the predominant classes, with mean values of 14.5% and 9.3%, respectively; the corresponding value for IgA was 5%. A comparable order of frequency (IgM greater than IgG greater than IgA) was observed for purified peripheral blood lymphocytes in the same and other control subjects. T cells comprised the majority (47%) of identifiable lung lymphocytes by the E rosette method. The presence of lung T cells was also corroborated by their proliferative response to mitogens (phytohemagglutinin and concanavallin A), but the response was less than that of equal numbers of peripheral blood lymphocytes from the same subjects. The B/T cell ratio for lung lymphocytes was comparable to results with peripheral blood lymphocytes in the same subjects, but a higher proportion of lung lymphocytes could not be identified as either T or B cells. It is postulated that lung lymphocytes participate in the local immune defenses of the lung.