MICROARRAY EXPRESSION PROFILE ANALYSIS OF LONG NONCODING RNAs IN PREMATURE BRAIN INJURY: A NOVEL POINT OF VIEW
MICROARRAY EXPRESSION PROFILE ANALYSIS OF LONG NONCODING RNAs IN PREMATURE BRAIN INJURY: A NOVEL POINT OF VIEW
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过早脑损伤中长非编码 RNA 的微阵列表达谱分析：一种新观点
DOI：
10.1016/j.neuroscience.2016.01.033
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发表时间：
2016-04-05
期刊：
影响因子：
3.3
通讯作者：
Lin, X. J.
中科院分区：
文献类型：
--
作者：
Chen, R. J.;Liu, L.;Lin, X. J.
长链非编码RNA（lncRNA）在中枢神经系统中丰富，并且在脑功能以及许多神经系统疾病中具有关键作用。然而，lncRNA在早产儿脑中的调节功能尚未得到很好的研究。本研究利用基因芯片技术研究了早产小鼠lncRNA的表达谱。共鉴定出1999个差异表达的lncRNA和955个差异表达的mRNA。基因本体论（GO）和通路分析表明，这些lncRNA参与神经系统发育和炎症反应等多种生物学过程。此外，构建lncRNA-mRNA-网络和TF-基因-lncRNA-network以鉴定核心调控lncRNA和转录因子。Y染色体性别决定区（SRY）基因可能是调控脑发育和脑损伤的关键转录因子。本研究首次揭示了早产儿脑组织中差异表达的lncRNA的表达谱，为探讨早产儿脑损伤的机制提供了新的视角。(C)2016年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Long noncoding RNAs (lncRNAs) are abundant in the central nervous system and have a key role in brain function as well as many neurological disorders. However, the regulatory function of lncRNAs in the premature brain has not been well studied. This study described the expression profile of lncRNAs in premature mice using microarray technology. 1999 differentially expressed lncRNAs and 955 differentially expressed mRNAs were identified. Gene Ontology (GO) and pathway analysis showed that these lncRNAs were involved in multiple biological processes, including the nervous system development and inflammatory response. Additionally, the lncRNA-mRNA-network and TF-gene-lncRNA-network were constructed to identify core regulatory lncRNAs and transcription factors. The sex-determining region of Y chromosome (SRY) gene may be a key transcription factor that regulates premature brain development and injury. This study for the first time represents an expression profile of differentially expressed lncRNAs in the premature brain and may provide a novel point of view into the mechanisms of premature brain injury. (C) 2016 IBRO. Published by Elsevier Ltd. All rights reserved.