A putative H(+)-K(+)-ATPase is selectively expressed in surface epithelial cells of rat distal colon.
A putative H(+)-K(+)-ATPase is selectively expressed in surface epithelial cells of rat distal colon.
复制标题
假定的H( )-K( )-ATP酶在大鼠远端结肠的表面上皮细胞中选择性表达。
DOI：
10.1152/ajpcell.1993.265.4.c1080
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Rossier,BC
中科院分区：
文献类型：
--
作者：
Jaisser,F;Coutry,N;Farman,N;Binder,HJ;Rossier,BC
最近，一个假定的远端结肠H(+)-K(+)-ATPaseα亚单位已被鉴定和鉴定(M.S.Crowson和G.E.Shull)。J.Biol.化学。267：13740-13748,1992年)。在本研究中，我们报道了这种可能的H(+)-K(+)-ATPase的组织和细胞表达。结果表明：首先，在肠道中，可能的H(+)-K(+)-ATPaseα亚基局限于结肠的远端，并且主要在表面上皮细胞中表达，而Na(+)-K(+)-ATPase的α1亚基也在腺体中表达。提示H(+)-K(+)-ATPaseα亚基是远端结肠终末分化的潜在标志物。第二，在子宫中，可能的H(+)-K(+)-ATPase局限于子宫肌层内和肌间带之间的区域，那里有非常丰富的血管供应和神经细胞。这种显著的表达提示H(+)-K(+)-ATPase可能不参与子宫液的pH和钾浓度的控制，而是参与血管和/或神经细胞的不同功能。第三，使用三种独立的不同方法(Northern印迹分析、核糖核酸酶保护分析和原位杂交)，我们无法在肾组织中检测到任何显著数量的H(+)-K(+)-ATPase转录本。我们的数据表明，推测的远端结肠H(+)-K(+)-ATPase可能与肾脏的亚型不同。最后，我们报道了一组简并的寡核苷酸序列，这些序列有助于在不同组织和不同物种中克隆Na(+)-K(+)-/H(+)-K(+)-ATPase基因家族的α-亚基。
Recently, a putative distal colon H(+)-K(+)-ATPase alpha-subunit has been identified and characterized (M. S. Crowson and G. E. Shull. J. Biol. Chem. 267:13740-13748, 1992). In the present study, we report the tissue and cell expression of this putative H(+)-K(+)-ATPase. The results indicate that, first, in the gut, the putative H(+)-K(+)-ATPase alpha-subunit is restricted to the distal part of the colon and is predominantly expressed in surface epithelial cells, in marked contrast to the alpha 1-subunit of Na(+)-K(+)-ATPase that is also expressed in glands. These data suggest that the H(+)-K(+)-ATPase alpha-subunit is a potential marker for terminal differentiation of distal colon. Second, in the uterus, the putative H(+)-K(+)-ATPase is restricted to the region of the myometrium between the inner and midmuscular zone that is very rich in vascular supply and nerve cells. This striking expression suggests that the H(+)-K(+)-ATPase may not be involved in the control of pH and potassium concentration of the uterine fluid but rather in distinct functions of vascular and/or nerve cells. Third, with the use of three independent and different approaches (Northern blot analysis, ribonuclease protection assay, and in situ hybridization), we were unable to detect any significant amount of H(+)-K(+)-ATPase transcripts in kidney tissue. Our data suggest that the putative distal colon H(+)-K(+)-ATPase is probably distinct from the kidney isoform. Finally, we report the sequence of a set of degenerate oligonucleotides that are useful to clone alpha-subunits of the Na(+)-K(+)-/H(+)-K(+)-ATPase gene family in different tissues and different species.