"Direct" detection and separation of DNA using nanoporous alumina filters
"Direct" detection and separation of DNA using nanoporous alumina filters
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DOI：
10.1021/la047959a
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发表时间：
2004-11-09
期刊：
影响因子：
3.9
通讯作者：
Germann, M
中科院分区：
文献类型：
--
作者：
Vlassiouk, I;Krasnoslobodtsev, A;Germann, M
使用氧化铝纳米多孔过滤器（AAO）与DNA修饰的“无标记”检测和分离/纯化的目标ss-DNA的概念被证明。DNA的高表面密度（4 × 10（12）cm（2））和高杂交效率（约100）。70%）与增加的有效表面积的组合使得该系统对于开发各种ss-DNA（或RNA）检测方法非常有吸引力。AAO在UV和IR区域中的中等透明度允许通过光学和IR吸收直接检测DNA杂交。观察到使用单次通过改进的过滤器结合来自溶液的靶ss-DNA的接近定量的效率。
The concept of using alumina nanoporous filters (AAO) modified with DNA for "label-free" detection and separation/purification of the target ss-DNA is demonstrated. The high surface density of DNA (4 x 10(12) cm(2)) and high efficiency of hybridization (ca. 70%) in combination with increased effective surface area make this system very attractive for development of various ss-DNA (or RNA) detection methods. Moderate transparency of AAO in the UV and IR regions allows direct detection of DNA hybridization by optical and IR absorption. Close to the quantitative efficiency of binding the target ss-DNA from solution using a single pass through the modified filter is observed.