Identification of erythropoietin-induced microRNAs in haematopoietic cells during erythroid differentiation
Identification of erythropoietin-induced microRNAs in haematopoietic cells during erythroid differentiation
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DOI：
10.1111/j.1365-2141.2008.07151.x
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发表时间：
2008-07-01
影响因子：
6.5
通讯作者：
Kato, Takashi
中科院分区：
文献类型：
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作者：
Kosaka, Nobuyoshi;Sugiura, Keiichi;Kato, Takashi
microRNAs（miRNAs）是一类非编码的小分子RNA，通过mRNA降解或翻译抑制来调控基因表达。miRNAs是否参与造血过程，如细胞分化、凋亡和维持足够水平的循环血细胞，尚未清楚阐明。使用人miRNA微阵列分析与其他因子依赖性UT-7细胞系相比，促红细胞生成素依赖性细胞系UT-7/EPO中的miRNA表达。在324种人类miRNAs中，MIRN 188、MIRN 362和MIRN 210的水平在UT-7/EPO细胞中显著升高，并且在UT-7细胞中用EPO刺激增加了这三种miRNAs的水平。值得注意的是，UT-7/EPO细胞中MIRN 210的敲低导致细胞凋亡。在小鼠胎肝细胞中，TER-119阳性细胞中MIRN 210的表达是TER-119阴性细胞中MIRN 210表达的两倍。MIRN 210的表达在体外红系成熟过程中升高。这些数据表明MIRN 210是一类新的调节miRNAs的成员，可能在红系成熟中发挥重要作用。
MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression through mRNA degradation or translation inhibition. It has not yet been clearly elucidated whether miRNAs participate in haematopoietic processes such as cell differentiation, apoptosis and maintenance of adequate levels of circulating blood cells. A human miRNA microarray was used to analyze miRNA expression in the erythropoietin-dependent cell line UT-7/EPO compared to other factor-dependent UT-7 cell lines. Among 324 human miRNAs, MIRN188, MIRN362 and MIRN210 levels were significantly elevated in UT-7/EPO cells, and stimulation with EPO in UT-7 cells increased the level of these three miRNAs. Notably, knockdown of MIRN210 in UT-7/EPO cells led to apoptosis. In mouse fetal liver cells, MIRN210 expression was twofold higher in TER-119-positive cells than in TER-119-negative cells. The expression of MIRN210 was elevated during erythroid maturation in vitro. These data suggest MIRN210 to be a member a new class of regulatory miRNAs that might play an important role in erythroid maturation.