Chondrogenic differentiation of human mesenchymal stem cells: a comparison between micromass and pellet culture systems
Chondrogenic differentiation of human mesenchymal stem cells: a comparison between micromass and pellet culture systems
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人间充质干细胞的软骨分化：微团和颗粒培养系统之间的比较
DOI：
10.1007/s10529-010-0293-x
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发表时间：
2010-09-01
影响因子：
2.7
通讯作者：
Huang, Dongsheng
中科院分区：
文献类型：
--
作者：
Zhang, Liangming;Su, Peiqiang;Huang, Dongsheng
高密度细胞培养是人骨髓间充质干细胞向软骨细胞分化的关键。两种高密度细胞培养系统，微团和沉淀培养，已被用来诱导hMSCs的软骨分化。在微团培养中，诱导软骨组织更大，更均匀，富含软骨特异性胶原蛋白II，但纤维软骨样特征，胶原蛋白I和肥大软骨细胞特征，胶原蛋白X，显着减少相比，那些在小球培养。真实的RT-PCR结果显示，微团培养中II型胶原和聚集蛋白聚糖mRNA表达上调，而X型胶原和I型胶原mRNA表达下调。因此，微团培养系统是体外软骨形成研究的一个有前途的工具。
High-density cell culture is pivotal for the chondrogenic differentiation of human mesenchymal stem cells (hMSCs). Two high-density cell culture systems, micromass and pellet culture, have been used to induce chondrogenic differentiation of hMSCs. In micromass culture, the induced-cartilage tissues were larger, more homogenous and enriched in cartilage-specific collagen II but the fibrocartilage-like feature, collagen I, and hypertrophic chondrocyte feature, collagen X, were markedly decreased compared to those in pellet culture. Furthermore, real time RT-PCR analysis demonstrated that collagen II and aggrecan mRNA were up-regulated while collagen X and collagen I mRNA were down-regulated in micromass culture. Thus, the micromass culture system is a promising tool for in vitro chondrogenic studies.