Comparative evaluation of three commercially available complement fixation test antigens for the diagnosis of glanders
Comparative evaluation of three commercially available complement fixation test antigens for the diagnosis of glanders
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DOI：
10.1136/vr.d5410
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发表时间：
2011-11-05
影响因子：
2.2
通讯作者：
Neubauer, H.
中科院分区：
文献类型：
--
作者：
Khan, I.;Wieler, L. H.;Neubauer, H.
通过对从腺流行区和非流行区采集的410份血清（随机采集的真阴性血清200份和实验免疫动物（12只兔，19匹马）采集的210份血清）进行检测，比较评价了c. c. pro （c. c. pro）、瓦赫宁根中央兽医研究所（CIDC）和美国农业部（USDA）三种市购补体固定试验（CFT）抗原的敏感性和特异性。临床阳性（135）和培养阳性（44）马，驴和骡子)。采用免疫印迹法（IB）作为金标准。CIDC抗原的敏感性最高（100%），其次是c. c. pro抗原（99.39%）。然而，USDA抗原显示显著低于(p
The sensitivity and specificity of three commercially available complement fixation test (CFT) antigens from c. c. pro (c. c. pro), Central Veterinary Institute of Wageningen UR (CIDC) and the United States Department of Agriculture (USDA) were comparatively evaluated by testing 410 sera collected from glanders-endemic and non-endemic areas (200 true-negative randomly collected sera and 210 sera collected from experimentally immunised animals (12 rabbits, 19 horses), clinically positive (135) and culture-positive (44) horses, donkeys and mules). Immunoblotting (IB) was used as the gold standard test. Highest sensitivity was shown for the CIDC antigen (100 per cent) followed by the c. c. pro antigen (99.39 per cent). However, the USDA antigen showed substantially less (p