Tumor cells interact with red blood cells via galectin-4-a short report
Tumor cells interact with red blood cells via galectin-4-a short report
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DOI：
10.1007/s13402-017-0317-9
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发表时间：
2017-08-01
影响因子：
6.6
通讯作者：
Bauer, Andrea S.
中科院分区：
文献类型：
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作者：
Helwa, Reham;Heller, Anette;Bauer, Andrea S.
背景肿瘤细胞的侵袭和转移能力与癌症进展的过程相关，因此，它代表了癌症治疗的障碍。到目前为止，关于循环肿瘤细胞和血细胞之间相互作用的信息有限。有充分证据表明，半乳糖凝集素-4在许多类型的肿瘤细胞中上调，并参与转移。方法将高表达galectin-4的结肠癌、正常胰腺癌和胰腺癌细胞株（n = 5）分别与不同供体的外周血细胞共同孵育。它们的相互作用和相关蛋白的免疫染色和活细胞imaging.Results检查，我们发现，（内源性或外源性）半乳糖凝集素-4表达的肿瘤细胞直接与红细胞相互作用。我们还观察到半乳糖凝集素-4和人血型抗原在这些细胞之间的接触部位的积累。通过比较附着于每个肿瘤细胞的RBC数量，我们发现具有高半乳糖凝集素-4预孵育表达水平的细胞比具有低表达水平的细胞附着显著更多的RBC（p < 1 × 10 - 7）。相反，我们发现，红细胞附着诱导半乳糖凝集素-4在肿瘤细胞中的表达。结论从我们的数据，我们得出结论，肿瘤细胞直接与红细胞通过半乳糖凝集素-4。
Background The ability of tumor cells to invade and metastasize is relevant to the process of cancer progression and, as such, it represents an obstacle to cancer cure. So far, limited information is available on interactions between circulating tumor cells and blood cells. It is well-documented that galectin-4 is upregulated in many types of tumor cells and is involved in metastasis. Here, we address the hypothesis that tumor cells may interact with red blood cells (RBCs) via galectin-4.Methods High galectin-4 expressing colon, normal pancreatic and pancreatic cancer-derived cell lines (n = 5) were incubated with peripheral blood cells from different donors. Their interactions and associated proteins were examined by immunostaining and live cell imaging.Results We found that (endogenous or exogenous) galectin-4 expressing tumor cells interact directly with RBCs. We also observed an accumulation of galectin-4 and human blood group antigens at the contact sites between these cells. By comparing the number of RBCs attaching to each tumor cell, we found that cells with high pre-incubation expression levels of galectin-4 attached significantly more RBCs than those with low expression levels (p < 1 x 10(-7)). Conversely, we found that RBC attachment induces galectin-4 expression in tumor cells.Conclusion From our data we conclude that tumor cells directly interact with red blood cells via galectin-4.