MALDI-TOF/TOF Mass Spectrometric Determination and Antioxidative Activity of Purified Phosphatidylcholine Fractions from Shrimp Species
MALDI-TOF/TOF Mass Spectrometric Determination and Antioxidative Activity of Purified Phosphatidylcholine Fractions from Shrimp Species
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MALDI-TOF/TOF 质谱测定虾纯化磷脂酰胆碱组分的抗氧化活性
DOI：
10.1021/acs.jafc.7b00217
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发表时间：
2017
影响因子：
6.1
通讯作者：
Zeng XX
中科院分区：
文献类型：
--
作者：
Zhou Li;Wang Yan;Wang Xiaolin;Liang Yi;Huang Zheng;Zeng Xiaoxiong;Zeng XX
研究了虾磷脂酰胆碱的分离纯化、性质及体外抗氧化活性。利用MALDI-TOF/TOF MS技术对虾PC的分子结构进行了分析，并对质子化和钠化PC前体产生的MS 2片段进行了鉴定。[M + Na -三甲胺]+、[M + Na - 205]+、[M + Na-RCOOH-三甲胺]+和[M + H-RCOOH-三甲胺]+等特异性片段可以区分前体类型，从而确定PC分子结构。采用DPPH自由基清除活性测定法评价了纯化虾PC组分的抗氧化活性，并通过监测氧化过程中过氧化值的变化，考察了纯化虾PC组分对山茶油氧化稳定性的影响。中国对虾和日本沼虾的PC组分具有较强的抗氧化活性。与对照组相比，0.2%（w/w）的虾PC均显著提高了山茶油的氧化稳定性（P< 0.05）。实验结果表明，虾PC可能是食用油或其他食品分散体的天然抗氧化剂的有价值的来源。
Purification, characterization, and antioxidative activity in vitro of shrimp phosphatidylcholines (PCs) were investigated. The molecular structures of shrimp PCs were determined by MALDI-TOF/TOF MS. The MS2fragments produced from protonated PC precursors and sodiated PC precursors were identified. The specific fragments including [M + Na – trimethylamine]+, [M + Na – 205]+, [M + Na – RCOOH – trimethylamine]+, and [M + H – RCOOH – trimethylamine]+could distinguish the precursor type to confirm PC molecular structures. The antioxidative activities of purified shrimp PC fractions were evaluated by assay of DPPH free radical scavenging activity, and their effects on the oxidative stability of camellia oil were measured by monitoring changes in the peroxide value assay during oxidation. The PC fractions fromPenaeus chinesisandMacrobranchium nipponenseshowed stronger antioxidative activities than those of other species. All of the shrimp PCs at 0.2% (w/w) improved the oxidative stability of camellia oil significantly (P< 0.05) compared to controls. The experimental findings suggest that shrimp PCs might be a valuable source of natural antioxidants for edible oils or other food dispersions.