Age-related changes in activities of mitochondrial electron transport complexes in various tissues of the mouse
Age-related changes in activities of mitochondrial electron transport complexes in various tissues of the mouse
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DOI：
10.1006/abbi.1999.1495
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发表时间：
2000-01-01
影响因子：
3.9
通讯作者：
Sohal, RS
中科院分区：
文献类型：
--
作者：
Kwong, LK;Sohal, RS
本研究的目的是研究线粒体在衰老过程中的作用，通过确定是否各种电子传递链氧化还原酶的活动在衰老过程中受到不利的影响，以及是否假设的年龄相关的改变在不同的组织遵循一个共同的模式。在分离自年轻（3.5个月）、成年（12-14个月）和老年（28-30个月）C57 BL/6小鼠的脑、心脏、骨骼肌、肝和肾的线粒体中测量呼吸复合物I、II、III和IV的活性。一些个别复合物的活动在老年动物中有所减少，但在各种组织中没有共同的模式。在一般情况下，活动的复合物受到更不利的影响，如脑，心脏和骨骼肌，其实质是由有丝分裂后的细胞，比那些在肝脏和肾脏，这是由缓慢分裂的细胞。年龄相关的潜在功能障碍性组织改变的主要特征是不同复合物之间活性比率的变化，这样它往往会阻碍线粒体有效地将电子转移到呼吸链的能力，从而对呼吸组分的氧化磷酸化和/或自氧化能力产生不利影响。(C)北京大学出版社.
The purpose of the present study was to examine the role of mitochondria in the aging process by determining whether the activities of various electron transport chain oxidoreductases are deleteriously affected during aging and whether the hypothesized age-related alterations in different tissues follow a common pattern. Activities of respiratory complexes I, II, III, and IV were measured in mitochondria isolated from brain, heart, skeletal muscle, Liver, and kidney of young (3.5 months), adult (12-14 months), and old (28-30 months) C57BL/6 mice. Activities of some individual complexes were decreased in old animals, but no common pattern can be discerned among various tissues. In general, activities of the complexes were more adversely affected in tissues such as brain, heart, and skeletal muscle, whose parenchyma is composed of postmitotic cells, than those in the liver and kidney, which are composed of slowly dividing cells. The main feature of age-related potentially dysfunctional alterations in tissues was the development of a shift in activity ratios among different complexes, such that it would tend to hinder the ability of mitochondria to effectively transfer electrons down the respiratory chain and thus adversely affect oxidative phosphorylation and/or autooxidizability of the respiratory components. (C) 2000 Academic Press.