The Population Genomics of Trans-Specific Inversion Polymorphisms in Anopheles gambiae
The Population Genomics of Trans-Specific Inversion Polymorphisms in Anopheles gambiae
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DOI：
10.1534/genetics.109.105817
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发表时间：
2009-09-01
期刊：
影响因子：
3.3
通讯作者：
Besansky, Nora J.
中科院分区：
文献类型：
--
作者：
White, Bradley J.;Cheng, Changde;Besansky, Nora J.
冈比亚按蚊染色体倒位的多态性在疟原虫适应环境变化中起着重要作用。最近，我们使用基于微阵列的发散映射结合有针对性的重测序映射2La倒位的替代安排之间的核苷酸分化。在这里，我们将相同的技术应用于四种不同的：An的2R染色体的多态性倒位。冈比亚。令人惊讶的是，分歧是低得多的替代安排所有2R反转相比，2La反转。对于重排之一，2Ru，我们成功地映射了一个非常小的区域（类似于100 kb）的升高的分歧。对于其他三个重排，我们没有发现任何地区的显着高分歧，尽管有足够的独立的证据，从自然种群的地理倾斜和季节性循环，并在实验室种群稳定的杂种优势多态性。如果这些倒位是假设的选择目标，我们建议：重排之间的分歧可能已经逃脱了检测线索保留祖先的多态性的情况下，最年轻的2R重排和广泛的基因流病的老2R倒位系统，隔离在两个AN。gambiae及其兄弟种An.阿拉伯的。
In the malaria mosquito Anopheles gambiae polymorphic chromosomal inversions clay play all important role in adaptation to environmental variation. Recently, we used microarray-based divergence mapping combined with targeted resequencing to map nucleotide differentiation between alternative arrangements of the 2La inversion. Here, we applied the same technique to four different: polymorphic inversions oil the 2R chromosome of An. gambiae. Surprisingly, divergence was much lower between alternative arrangements for all 2R inversions when compared to the 2La inversion. For one of the rearrangements, 2Ru, we successfully mapped a very small region (similar to 100 kb) of elevated divergence. For the other three rearrangements, we did not identify any regions of significantly high divergence, despite ample independent evidence from natural populations of geographic clines and seasonal cycling, and stable heterotic polymorphisms in laboratory populations. If these inversions are the targets of selection as hypothesized, we suggest that: divergence between rearrangements may have escaped detection clue to retained ancestral polymorphism in the case of the youngest 2R rearrangements and to extensive gene flux ill the older 2R inversion systems that segregate in both An. gambiae and its sibling species An. arabiensis.