The expression of thioredoxin-1 and inflammatory cytokines in patients with sepsis
The expression of thioredoxin-1 and inflammatory cytokines in patients with sepsis
复制标题
脓毒症患者硫氧还蛋白-1及炎性细胞因子的表达
DOI：
10.1080/08923973.2020.1755309
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发表时间：
2020-05-03
影响因子：
3.3
通讯作者：
Chen, Guobing
中科院分区：
文献类型：
--
作者：
Shao, Ruifei;Yang, Yan;Chen, Guobing
背景：脓毒症是由宿主对感染的失调反应引起的危及生命的器官功能障碍。炎症反应和氧化应激在脓毒症的病理生理过程中起重要作用。硫氧还蛋白 - 1（Trx - 1）是一种广泛存在的小分子巯基蛋白，具有与脓毒症发病机制相关的氧化还原/炎症调节特性。因此，我们研究了脓毒症患者外周血中Trx - 1、炎症因子和氧化应激的表达水平及其意义，并探讨Trx - 1与炎症因子和氧化应激的关系。
方法：收集脓毒症患者和健康对照者的血浆样本。采用酶联免疫吸附测定（ELISA）检测人血浆样本中的白细胞介素（IL - 1β）、IL - 6、肿瘤坏死因子（TNF - α）、E - 选择素、内皮素 - 1（ET - 1）、硫氧还蛋白 - 1、C - 反应蛋白（CRP）、降钙素原（PCT）；采用逆转录 - 聚合酶链反应（RT - PCR）检测Trx - 1和硫氧还蛋白相互作用蛋白（TXNIP）的mRNA水平。采用比色法测定脓毒症患者外周血中谷胱甘肽（GSH）和丙二醛（MDA）的表达水平；采用急性生理与慢性健康状况评分系统Ⅱ（APACHE Ⅱ）评估疾病严重程度。
结果：脓毒症患者血浆中Trx - 1、炎症因子和氧化应激的表达水平显著升高，TXNIP则相反。
结论：我们的结果表明，Trx - 1在脓毒症患者的炎症和氧化应激中起重要作用。Trx - 1可能是脓毒症的一个潜在治疗靶点。
Background: Sepsis is life-threatening organ dysfunction caused by a dysregulated host response to infection. Inflammatory response and oxidative stress play an important role in the pathophysiological process of sepsis. Thioredoxin-1 (Trx-1) is a small ubiquitous thiol protein with redox/inflammation modulatory properties relevant to the pathogenesis of sepsis. We therefore investigated the expression level and significance of Trx-1, inflammatory factors and oxidative stress in peripheral blood of sepsis patients, and to explore Trx-1 relationship with inflammatory factors and oxidative stress. Methods: Plasma samples were collected from patients with sepsis and those with healthy control. Enzyme-linked immunosorbent assays (ELISA) were used to detect for interleukin (IL-1 beta), IL-6, tumor necrosis factor (TNF-alpha), E-selectin, endothelin-1 (ET-1), thioredoxin-1, C-reactive protein (CRP), procalcitonin (PCT) for human plasma samples; RT-PCR detection of Trx-1 and thioredoxin-interacting protein (TXNIP) mRNA levels. Colorimetric assay for glutathione (GSH) and malondialdehyde (MDA) expression level in peripheral blood of patients with sepsis; Disease severity was assessed as APACHE II. Results: The expression levels of Trx-1, inflammatory factors and oxidative stress in plasma of patients with sepsis were significantly increased, TXNIP opposite. Conclusion: Our results show that Trx-1 play important role in inflammation and oxidative stress in sepsis patients. Trx-1 may be a potential therapeutic target in sepsis.