CELL-LINEAGE PATTERNS IN THE SHOOT APICAL MERISTEM OF THE GERMINATING MAIZE EMBRYO
CELL-LINEAGE PATTERNS IN THE SHOOT APICAL MERISTEM OF THE GERMINATING MAIZE EMBRYO
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DOI：
10.1007/bf00402877
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发表时间：
1988-07-01
期刊：
影响因子：
4.3
通讯作者：
POETHIG, RS
中科院分区：
文献类型：
--
作者：
MCDANIEL, CN;POETHIG, RS
玉米茎尖分生组织的命运图。通过检查由γ-葡聚糖诱导的体细胞区段（克隆）来构建射线芽顶端分生组织产生茎，叶，和生殖结构以上叶6发芽后，这里的分析涉及它们的形成。在160个已产生17或18片叶的成年植物中，对277个花青素苷缺乏的扇区的大小和位置进行评分。在穗芽或雄穗上发现的扇区通常延伸到植物的营养部分。扇形的长度范围从1到6个节间，在植株的所有位置都观察到了多于1个节间的扇形。单节间节段在基部节间（7，8，9）占优势，而较长的节段在中部和上部节间常见。产生特定节间的细胞的表观数量是可变的，并且扇区不限于谱系单位：叶，其下方的节间，以及节间基部的腋芽和腋芽。这些观察建立了分生组织活动的两个主要特征：1）在萌发时，任何细胞或细胞群的发育命运都不是固定的，以及2）在萌发时，分生组织中相同位置的细胞可以在成年植物中产生非常不同数量的组织。因此，萌发分生组织中特定细胞的发育命运只能以一般的方式分配。
A fate map for the shoot apical meristem of Zea mays L. at the time of germination was constructed by examining somatic sectors (clones) induced by .gamma.-rays. The shoot apical meristem produced stem, leaves, and reproductive structures above leaf 6 after germination and the analysis here concerns their formation. Of 160 adult plants which had produced 17 or 18 leaves, 277 anthocyanin-deficient sectors were scored for size and position. Sectors found on the ear shoot or in the tassel most often extended into the vegetative part of the plant. Sectors ranged from one to six internodes in length and some sectors of more than one internode were observed at all positions on the plant. Single-internode sectors predominated in the basal internodes (7, 8, 9) while longer sectors were common in the middle and upper internodes. The apparent number of cells which gave rise to a particular internode was variable and sectors were not restricted to the lineage unit: a leaf, the internode below it, and the axillary bud and prophyll at the base of the internode. These observations established two major features of meristem activity: 1) at the time of germination of developmental fate of any cell or group of cells was not fixed, and 2) at the time of germination cells at the same location in a meristem could produce greatly different amounts of tissue in the adult plant. Consequently, the developmental fate of specific cells in the germinating meristem could only be assigned in a general way.