CROSS-REACTIONS BETWEEN ACTINOBACILLUS (HAEMOPHILUS) PLEUROPNEUMONIAE STRAINS OF SEROTYPE-1 AND SEROTYPE-9
CROSS-REACTIONS BETWEEN ACTINOBACILLUS (HAEMOPHILUS) PLEUROPNEUMONIAE STRAINS OF SEROTYPE-1 AND SEROTYPE-9
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DOI：
10.1128/jcm.28.3.535-539.1990
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发表时间：
1990-03-01
影响因子：
9.4
通讯作者：
MITTAL, KR
中科院分区：
文献类型：
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作者：
MITTAL, KR
用凝集试验、凝集试验(CoA)、间接血凝试验(IHA)、Co-IHA试验、环状沉淀试验(RP)和免疫扩散试验(ID)对1、9型胸膜肺炎放线杆菌的血清学特性进行了研究。未加热和加热处理的细菌细胞的颗粒抗原和可溶性抗原用于各种血清学试验。凝集试验、CoA试验和RP试验证实了血清型1和9型毒株之间的共同抗原。CoA、RP和ID试验对鉴别血清型1和9型毒株的特异性抗原活性有帮助。用未加热或加热的全细胞盐水提取物致敏的绵羊红细胞的IHA和Co-IHA试验以及以煮熟的全细胞盐水提取物为抗原的ID试验区分了1型和9型毒株。在用异种全细胞抗原对兔抗血清吸收的研究中，试管凝集试验和IHA试验没有发现抗体吸收，表明1型和9型毒株属于两个不同的血清群。血清1型和9型之间的交叉反应可能是由于与细胞壁抗原相关的共同表位所致。
Actinobacillus pleuropneumoniae strains of serotypes 1 and 9 were studied for their serological properties by means of agglutination, coagglutination (CoA), indirect hemagglutination (IHA), Co-IHA, ring precipitation (RP), and immunodiffusion (ID) tests. Particulate and soluble antigens of unheated and heat-treated bacterial cells were used in various serological tests. Agglutination, CoA, and RP tests demonstrated common antigens between strains of serotypes 1 and 9. Quantitative estimation of serotype-specific antigenic activity by CoA, RP, and ID tests proved useful in differentiating strains of serotypes 1 and 9. IHA and Co-IHA tests using sheep erythrocytes sensitized with unheated or heat-treated whole-cell saline extract and the ID test using boiled whole-cell saline extract as antigen distinguished the strains of serotypes 1 and 9. In studies of absorption of rabbit antisera with heterologous whole-cell antigens there was no absorption of antibodies in tube agglutination and IHA tests, suggesting that serotype 1 and 9 strains belong to two distinct serogroups. It appears that the cross-reactivity between serotype 1 and 9 strains could be due to common epitopes associated with cell wall antigens.