Identification of cancer stem-like cells in the C6 glioma cell line and the limitation of current identification methods
Identification of cancer stem-like cells in the C6 glioma cell line and the limitation of current identification methods
复制标题
C6胶质瘤细胞系中癌症干细胞样细胞的鉴定及当前鉴定方法的局限性
DOI：
10.1007/s11626-008-9115-z
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发表时间：
2008-08-01
影响因子：
2.1
通讯作者：
Yang, Xiaofeng
中科院分区：
文献类型：
--
作者：
Shen, Gang;Shen, Fang;Yang, Xiaofeng
肿瘤干细胞(CSC)假说为脑肿瘤的发生和复发提供了新的思路。特异性识别和靶向清除肿瘤内的这些CSCs是治疗难治性脑肿瘤的一种有前途的治疗策略。在本研究中，我们尝试通过三种不同的鉴定方法来鉴定大鼠C6胶质瘤细胞系中的CSCs。值得注意的是，单细胞克隆分析表明，大多数C6细胞是具有自我更新、体外多向分化潜能和体内致瘤能力的肿瘤干细胞样细胞。令人惊讶的是，CD133未能确定C6系中总的癌症干细胞样细胞群，因为CD133(+)和CD133(-)C6细胞都有癌症干细胞样细胞组分。此外，用于流式细胞仪分离侧群(SP)的Hoechst 33342染色被发现对C6细胞有害。因此，CD133(-)和非SP C6细胞也可能含有肿瘤干细胞。这些结果暗示了在C6品系中使用现有鉴定方法的局限性，并强调了确定CSCs的遗传和分子基础的重要性。
The cancer stem cell (CSC) hypothesis has provided insights into the initiation and recurrence of brain tumor. Specific identification and targeted elimination of these CSCs within the tumor mass represents a promising therapeutic strategy for refractory brain tumors. In this study, we attempted to identify CSCs in the rat C6 glioma cell line by three different identification methods. It is interesting to note that single-cell clonal analysis showed most C6 cells are cancer stem-like cells with characteristics of self-renewal, multilineage differentiation potentials in vitro, and tumorigenic capacity in vivo. It is surprising to note that CD133 failed to identify the total cancer stem-like cell population in the C6 line, since both CD133 (+) and CD133 (-) C6 cells have cancer stem-like cell fractions. Moreover, Hoechst 33342 staining, which is used in flow cytometry to isolate the side population (SP), was found to be harmful to C6 cells. Therefore, CD133 (-) and non-SP C6 cells may also harbor cancer stem-like cells. These results imply the limitation of using current identification methods in C6 line and underscore the importance of defining the genetic and molecular basis of CSCs.