Elevated environmental temperature enhances immunity in experimental Chagas' disease.
Elevated environmental temperature enhances immunity in experimental Chagas' disease.
复制标题
环境温度升高可增强实验性恰加斯病的免疫力。
DOI：
10.1128/iai.57.1.13-17.1989
复制
发表时间：
1989
影响因子：
3.1
通讯作者：
Kuhn,RE
中科院分区：
文献类型：
--
作者：
Anderson,KJ;Kuhn,RE
C3H小鼠对巴西株克氏锥虫高度易感。这些小鼠通常在感染的急性期死亡，并对异源和寄生虫抗原产生严重的免疫抑制。在这项研究中，我们证实了早期的报告，即感染小鼠保持在升高的环境温度（36摄氏度）显着更耐T。cruzi比20至24摄氏度的小鼠更容易存活。为了确定环境温度升高的益处是否是由于宿主免疫系统的改变，在未感染和T.克氏病毒感染的小鼠用绵羊红细胞（SRBC）或三硝基苯基（TNP）与T. cruzi，并测定脾直接空斑形成细胞（DPFC）对SRBC和对TNP缀合的SRBC的反应。在升高的环境温度下，感染小鼠对SRBC的DPFC反应远高于室温下感染小鼠的抑制反应，略高于年龄匹配的未感染对照小鼠的反应。同样地，将感染的小鼠维持在36 ° C显著增强了T辅助细胞的寄生虫特异性应答，如用TNP缀合的TC免疫的小鼠的抗TNP DPFC应答所反映的。
C3H mice are highly susceptible to the Brazil strain of Trypanosoma cruzi. These mice usually die during the acute phase of infection and develop a profound immunosuppression to heterologous and parasite antigen. In this study, we confirmed earlier reports that infected mice maintained at elevated environmental temperature (36 degrees C) are significantly more resistant to T. cruzi than are mice kept at 20 to 24 degrees C. To determine whether the benefits of increased environmental temperature were due to alterations in the host immune system, the production of antibody to heterologous antigen and the development of parasite-specific T-helper cells were examined in noninfected and T. cruzi-infected mice. Mice were immunized with either sheep erythrocytes (SRBC) or trinitrophenyl groups (TNP) conjugated to fixed culture forms of T. cruzi, and the splenic direct plaque-forming cell (DPFC) responses to SRBC and to TNP-conjugated SRBC were determined. The DPFC response to SRBC from infected mice maintained at elevated environmental temperature was much higher than the suppressed response of infected mice held at room temperature and slightly higher than the response of age-matched noninfected control mice. Likewise, maintaining infected mice at 36 degrees C significantly enhanced the parasite-specific responses of T-helper cells, as reflected by anti-TNP DPFC responses of mice immunized with TNP-conjugated TC.