Molecular and morphological characterization of heterorhabditid entomopathogenic nematodes from the tropical rainforest in Brazil
Molecular and morphological characterization of heterorhabditid entomopathogenic nematodes from the tropical rainforest in Brazil
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DOI：
10.1590/s0074-02762008000200005
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发表时间：
2008-03-01
期刊：
影响因子：
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通讯作者：
Winter, Carlos E
中科院分区：
文献类型：
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作者：
Dolinski, Claudia;Kamitani, Fernando L;Winter, Carlos E
尽管原始森林大量消失，亚马逊雨林仍然是生物多样性的一个极其丰富的来源。近年来，昆虫病原线虫（entomopathogenic nematodes，EPN）已从世界各地的土壤中分离出来，并成功地用作许多害虫的生物防治剂。因此，在巴西Rondonia的Monte Negro的雨林中进行取样，目的是发现EPN的新菌株和/或种，用于未来开发作为生物防治剂。从9个采样点采集的156个土样中分离到19个菌株，均属于异小杆属。四个菌株进行了详细的形态和分子评价。根据形态计量学和内转录间隔区（ITS）序列数据，菌株LPP 1，LPP 2和LPP 4被鉴定为印度异小杆线虫，而LPP 7被认为是鲍氏异小杆线虫。对H. indica和H. baujardi分离物显示限制性内切酶Tth 111的多态性位点，该位点可用于区分这两个物种。因此，菌株LPP 1、LPP 2、LPP 3、LPP 4和LPP 9被鉴定为H. LPP 5、LPP 7、LPP 8和LPP 10被鉴定为H. baujardi。
Despite massive losses of primary forest, the Amazonian rainforest remains an extremely rich source of biodiversity. In recent years, entomopathogenic nematodes (EPNs) have been isolated from soil in various parts of the world and used successfully as biological control agents against numerous insect pests. Therefore, a sampling in the rainforest of Monte Negro, Rondonia, Brazil was conducted with the aim of discovering new strains and/or species of EPNs for future development as biological control agents. From 156 soil samples taken at nine collecting sites, 19 isolates were obtained, all of them belonging to the genus Heterorhabditis. Four strains were subjected to detailed morphological and molecular evaluation. Based on morphometrics and internal transcribed spacer (ITS) sequence data, the strains LPP1, LPP2 and LPP4 were identified as Heterorhabditis indica, whereas LPP7 was considered Heterorhabditis baujardi. Comparative analysis of the ITS1 sequence of H. indica and H. baujardi isolates showed a polymorphic site for the restriction enzyme Tth 111 that could be used to distinguish the two species. Consequently, strains LPP1, LPP2, LPP3, LPP4, and LPP9 were identified as H. indica, whereas LPP5, LPP7, LPP8 and LPP10 were identified as H. baujardi.