Osteogenic ability of rat bone marrow concentrate is at least as efficacious as mesenchymal stem cells in vitro.
Osteogenic ability of rat bone marrow concentrate is at least as efficacious as mesenchymal stem cells in vitro.
复制标题
在体外，大鼠骨髓浓缩物的成骨能力至少与间充质干细胞一样有效。
DOI：
10.1002/jbm.b.34340
复制
发表时间：
2019
期刊：
影响因子：
--
通讯作者：
Goodman,StuartB
中科院分区：
文献类型：
--
作者：
Kohno,Yusuke;Lin,Tzuhua;Pajarinen,Jukka;Romero-Lopez,Monica;Maruyama,Masahiro;Huang,Jhih-Fong;Nathan,Karthik;Yao,Zhenyu;Goodman,StuartB
使用骨髓浓缩物（BMC）或纯化和扩增的间充质干细胞（MSCs）进行细胞治疗已被证明具有很好的成骨能力。然而，很少有研究直接比较它们的相对成骨能力。本研究的目的是比较密度梯度离心分离的BMC与玻璃化3月龄Sprague-Dawley大鼠细胞的骨髓源间充质干细胞的成骨能力。将分离的细胞接种到24孔板上（1 × 105个细胞/孔），并在对照生长培养基、含地塞米松的成骨培养基或不含地塞米松的培养基中培养（模拟其体内组织环境）。在第2周评估碱性磷酸酶活性，在第4周评估骨钙素使用定量实时聚合酶链反应，在第4周评估茜素红染色。在含有地塞米松的成骨培养基中，BMC显示出与MSCs相当（骨钙素）甚至更强（茜素红染色）的成骨能力，这表明BMC中各种细胞之间的交叉对话导致了更大的成骨能力。此外，在没有地塞米松的成骨培养基中，BMC显示出与单独的MSCs相同的（骨钙素）或更大的（茜素红染色）骨形成趋势。我们的研究结果表明，BMC至少具有与MSCs相当的骨再生潜力。©2019 Wiley期刊公司[J]中国生物医学工程学报，2016,31 (1):1 - 4
Cell therapy using bone marrow concentrate (BMC) or purified and expanded mesenchymal stem cells (MSCs) has been shown to have a promising osteogenic capacity. However, few studies have directly compared their relative osteogenic ability. The aim of this study was to compare the osteogenic ability of BMC isolated by density gradient centrifugation with bone marrow‐derived MSCsin vitrousing the cells of 3‐month‐old Sprague–Dawley rats. The isolated cells were seeded onto 24‐well plates (1 × 105cells/well) and cultured in control growth media, osteogenic media with dexamethasone, or media without dexamethasone (which simulated thein vivotissue environment). Alkaline phosphatase activity at week 2, osteocalcin using quantitative real‐time polymerase chain reaction at week 4, and Alizarin red staining at week 4 were evaluated. In the osteogenic media with dexamethasone, BMC showed equivalent (osteocalcin) or even greater (Alizarin red staining) osteogenic ability compared to MSCs, suggesting that cross‐talk among various cells in the BMC leads to greater osteogenesis. Furthermore, in the osteogenic media without dexamethasone, BMC showed equivalent (osteocalcin) or a trend for greater (Alizarin red staining) bone formation than MSCs alone. Our results suggest that BMC has at least comparable bone regeneration potential to MSCs. © 2019 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 107B:2500–2506, 2019