Composition and distribution of cell wall phenolic compounds in flax (Linum usitatissimum L.) stem tissues
Composition and distribution of cell wall phenolic compounds in flax (Linum usitatissimum L.) stem tissues
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DOI：
10.1006/anbo.1999.1091
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发表时间：
2000-04-01
期刊：
影响因子：
4.2
通讯作者：
Lozovaya, VV
中科院分区：
文献类型：
--
作者：
Gorshkova, TA;Salnikov, VV;Lozovaya, VV
采用生物化学、组织化学和超微结构方法，对棒茎木质部和韧皮部富含纤维的果皮中的细胞壁酚类化合物进行了分析。利用漆酶的酶-金法对细胞壁酚类物质进行了定位，揭示了几种金颗粒的分布模式。其中一种主要类型（均匀分布的单个金颗粒）主要存在于木质部中，而另一种类型（10 -40个金颗粒的紧密分支群）则同时存在于木质部和纤维细胞中。木质素含量的茎部分估计Klason程序和巯基乙酸测定，和酚类产品回收后，碱性氧化铜氧化细胞壁进行了GC分析。通过结合化学分析数据和组织内各种金颗粒类型的频率，不同的金颗粒分布模式可以归因于某些细胞壁酚类化合物;木质素被染色为均匀分布的单个金颗粒，而分支簇代表羟基肉桂酸。Klason程序没有从亚麻纤维中除去所有非木质素组分，以其高度结晶的纤维素而闻名，并且大大高估了木质素含量。巯基乙酸含量测定结果与GC和显微镜观察结果一致。(C)2000年《植物学年鉴》（Annals of Botany Company）
Cell wall phenolic compounds were analysed in xylem and bast fibre-rich peels of Bar stems by biochemical, histochemical and ultrastructural approaches. Localization of cell wall phenolics by the enzyme-gold method using laccase revealed several gold particle distribution patterns. One of the major types (an even distribution of single gold particles) was present mainly in xylem, while the other (compact branched groups of ten-40 gold particles) was found both in xylem and fibre cells. The lignin content of the stem parts was estimated by the Klason procedure and by the thioglycolic acid assay, and the phenolic products recovered after alkaline cupric oxide oxidation of cell walls were analysed by GC. By combining chemical analysis data and the frequency of various gold particle types within the tissues, different patterns of gold particle distribution could be ascribed to certain cell wall phenolics; lignin was stained as evenly distributed single gold particles, while branched clusters represented hydroxycinnamic acids. The Klason procedure did not remove all the non-lignin components from flax fibres, known for their highly crystalline cellulose, and considerably overestimated the lignin content. The thioglycolic acid assay results were consistent with GC and microscopic observations. (C) 2000 Annals of Botany Company.