Responses of blood mononucleated cells and clinical outcome of non-celiac gluten sensitive pediatric patients to various cereal sources: a pilot study
Responses of blood mononucleated cells and clinical outcome of non-celiac gluten sensitive pediatric patients to various cereal sources: a pilot study
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DOI：
10.1080/09637486.2017.1315058
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发表时间：
2017-01-01
影响因子：
3.9
通讯作者：
Spisni, Enzo
中科院分区：
文献类型：
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作者：
Alvisi, Patrizia;De Fazio, Luigia;Spisni, Enzo
非腹腔麸质敏感性（NCGS）是一个临床实体最近记录的科学界在儿科患者。然而，其触发机制在很大程度上仍未确定。我们研究了11名NCGS儿童，这些儿童是在排除乳糜泻（CD）和小麦过敏后，根据小麦摄入量与症状发展之间的明确关系诊断的，与18名活动性CD儿童相匹配。16名儿科患者也被招募作为对照。从NCGS、CD和对照患者获得的培养的外周血单核细胞（PBMC）在从古代和现代品种提取的小麦蛋白的存在下培养。结果表明，小麦蛋白诱导NCGS儿科患者PBMC中促炎趋化因子CXCL10的过度活化，并且这种过度表达还依赖于从中提取蛋白质的小麦品种。从现代小麦品种蛋白激活CXCL10在更大程度上比那些提取从古老的小麦基因型。
Non-celiac gluten sensitivity (NCGS) is a clinical entity recently documented by the scientific community in pediatric patients. Nevertheless, its triggering mechanisms remain largely unsettled. We studied 11 children with NCGS who were diagnosed based on a clear-cut relationship between wheat consumption and development of symptoms, after excluding celiac disease (CD) and wheat allergy, matched with 18 children with active CD. Sixteen pediatric patients were also enrolled as controls. Cultured peripheral blood mononucleated cells (PBMCs) obtained from NCGS, CD and control patients were cultured in the presence of wheat proteins extracted from ancient and modern cultivars. Results demonstrated that wheat proteins induced an overactivation of the proinflammatory chemokine CXCL10 in PBMC from NCGS pediatric patients and that this overexpression also depended on the wheat cultivar from which proteins were extracted. Proteins from modern wheat cultivar activated CXCL10 to a greater extent than those extracted from ancient wheat genotypes.