Large scale infrared imaging of tissue micro arrays (TMAs) using a tunable Quantum Cascade Laser (QCL) based microscope
Large scale infrared imaging of tissue micro arrays (TMAs) using a tunable Quantum Cascade Laser (QCL) based microscope
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DOI：
10.1039/c4an00638k
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发表时间：
2014-08-21
期刊：
影响因子：
4.2
通讯作者：
Gardner, Peter
中科院分区：
文献类型：
--
作者：
Bassan, Paul;Weida, Miles J.;Gardner, Peter
振动光谱学领域的化学成像正在发展成为补充数字组织病理学的有前途的工具。应用包括通过基于光谱的化学信息自动识别组织/细胞类型和疾病状态来筛选活检组织。为了集成到临床实践中，需要加快数据采集速度，以实现基于机架的系统，在该系统中，样本被快速成像，以与当前的可见扫描仪竞争，在该可见扫描仪中，可以在一夜之间扫描数百张载玻片。目前傅里叶变换红外（FTIR）成像与焦平面阵列（FPA）探测器是目前最先进的仪器红外吸收化学成像，但最近的发展，广泛可调谐激光器在中红外范围被认为是最有前途的潜在候选人为下一代显微镜。在本文中，我们测试了一个原型量子级联激光器（QCL）的光谱成像显微镜的重点是离散频率化学成像。我们演示了如何在9分钟内测量包含200个核心的2 x 2.4 cm（2）乳腺组织微阵列（TMA）的酰胺I带（1655 cm（-1））的蛋白质化学图像。这一结果表明，需要来自几个关键波长的化学图像的应用程序将理想地由基于激光的显微镜提供服务。
Chemical imaging in the field of vibrational spectroscopy is developing into a promising tool to complement digital histopathology. Applications include screening of biopsy tissue via automated recognition of tissue/cell type and disease state based on the chemical information from the spectrum. For integration into clinical practice, data acquisition needs to be speeded up to implement a rack based system where specimens are rapidly imaged to compete with current visible scanners where 100's of slides can be scanned overnight. Current Fourier transform infrared (FTIR) imaging with focal plane array (FPA) detectors are currently the state-of-the-art instrumentation for infrared absorption chemical imaging, however recent development in broadly tunable lasers in the mid-IR range is considered the most promising potential candidate for next generation microscopes. In this paper we test a prototype quantum cascade laser (QCL) based spectral imaging microscope with a focus on discrete frequency chemical imaging. We demonstrate how a protein chemical image of the amide I band (1655 cm(-1)) of a 2 x 2.4 cm(2) breast tissue microarray (TMA) containing over 200 cores can be measured in 9 min. This result indicates that applications requiring chemical images from a few key wavelengths would be ideally served by laser-based microscopes.