Upregulation of glucose-6-phosphate dehydrogenase and NAD(P)H oxidase activity increases oxidative stress in failing human heart
Upregulation of glucose-6-phosphate dehydrogenase and NAD(P)H oxidase activity increases oxidative stress in failing human heart
复制标题
DOI：
10.1016/j.cardfail.2007.04.003
复制
发表时间：
2007-08-01
影响因子：
6
通讯作者：
Gupte, Sachin A.
中科院分区：
文献类型：
--
作者：
Gupte, Rakhee S.;Vijay, Venkataramana;Gupte, Sachin A.
背景：我们之前发现，在扩张型心肌病的狗模型中，葡萄糖-6-磷酸脱氢酶 (G6PD) 产生的较高 NADPH 水平可以增强 NAD(P)H 氧化酶产生的心肌超氧化物。因此，我们测试了缺血性心肌病心力衰竭患者的 G6PD 活性是否升高、NADPH 水平是否增加以及 NAD(P)H 氧化酶衍生的超氧化物产生增加。方法和结果：从 8 例接受心室修复手术的充血性心力衰竭患者中收集左心室手术废弃物，而对照左心室组织从 5 颗被认为不适合移植的正常供体心脏中获得。在组织匀浆中进行生化测定。我们发现，与正常心脏相比，衰竭心脏中的超氧化物和过氧化氢分别升高了 9 倍和 3 倍（P
Background: We previously found that higher NADPH levels produced by glucose-6-phosphate dehydrogenase (G6PD) can enhance myocardial superoxide generation by NAD(P)H oxidase in a dog model of dilated cardiomyopathy. Therefore, we tested whether G6PD activity is elevated and enhances NADPH level and increases NAD(P)H oxidase-derived superoxide production in the myocardium from patients with heart failure from ischemic cardiomyopathy.Methods and Results: Surgical discards of left ventricle were collected from 8 congestive heart failure patients undergoing surgical ventricular restoration procedures, whereas control left ventricle tissue was obtained from 5 normal donor hearts deemed not suitable for transplantation. Biochemical assays were performed in tissue homogenates. We found that superoxide and hydrogen peroxide were elevated, respectively, by 9- and 3-fold in failing versus normal hearts (P