Diversity of Hepatic Stem Cells in the Fetal and Adult Liver
Diversity of Hepatic Stem Cells in the Fetal and Adult Liver
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DOI：
10.1055/s-2004-815557
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发表时间：
2003
影响因子：
4.2
通讯作者：
Yun-Wen Zheng1,2;Hideki Taniguchi2
中科院分区：
文献类型：
--
作者：
Yun-Wen Zheng1,2;Hideki Taniguchi2
肝脏是一个具有巨大再生能力的器官。肝干细胞经流式细胞仪分选和单细胞鉴定为c-Met + CD 49 f +/low c-Kit -CD 45-TER 119-细胞，具有多向分化潜能和自我更新能力。这些细胞可以在体外和体内分化成一些上皮细胞，这些上皮细胞能够在适当的移植后重建肝脏、胰腺和肠内的组织。分离的胎儿肝干细胞的特征还在于能够在发育中的肝脏中维持其自我更新能力。因此，这些细胞可能是将治疗基因递送到肝脏的理想载体。我们还研究了胚胎肝干细胞和肝卵圆细胞在成人肝脏之间的关系。这两个亚型共享的多潜能分化能力和表面标志物表达谱，表明肝卵圆细胞可能是胎儿肝干细胞的后代。我们还讨论了肝细胞再增殖、外源性肝干细胞和转分化的相关主题，从肝干细胞的基本特性和全面描述到该领域的最新进展，在这篇简短的文章中讨论了我们最近的研究。
The liver is an organ with tremendous regenerative capacity. Hepatic stem cells, identified by flow cytometric sorting and single cell–based assays as c-Met + CD49f +/low c-Kit – CD45 – TER119 – cells in murine fetal livers, possess multilineage differentiation potential and self-renewing capability. These cells can differentiate in vitro as well as in vivo into some epithelia capable of reconstituting tissues within the liver, pancreas, and intestine following appropriate transplantation. The isolated fetal hepatic stem cells are also characterized by the ability to maintain their self-renewal capability in the developing liver. Thus, these cells may be ideal vehicles for delivering therapeutic genes to the liver. We also examined the relationship between fetal hepatic stem cells and hepatic oval cells in the adult liver. These two subtypes shared both multipotential capabilities of differentiation and surface marker expression profiles, suggesting that hepatic oval cells are probably the progeny of fetal hepatic stem cells. We also address the related topics of hepatocyte re-population, exogenous hepatic stem cells, and transdifferentiation.Topics ranging from the fundamental properties and comprehensive descriptions of hepatic stem cells to current progress in the field are discussed in this brief article and our recent studies are addressed.