Aqueous chromatography system using pH- and temperature-responsive stationary phase with ion-exchange groups
Aqueous chromatography system using pH- and temperature-responsive stationary phase with ion-exchange groups
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DOI：
10.1016/j.chroma.2006.01.068
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发表时间：
2006-06-30
影响因子：
4.1
通讯作者：
Okano, Teruo
中科院分区：
文献类型：
--
作者：
Ayano, Eri;Nambu, Kyoko;Okano, Teruo
本文报道了布洛芬、酮洛芬和萘普生等非甾体抗炎药(NSAIDs)等非甾体抗炎药(NSAIDs)等非甾体抗炎药(NSAIDs)的高效液相色谱分析技术。在本研究中，我们设计了一种新型的N-异丙基丙烯酰胺(NIPAAm)、甲基丙烯酸丁酯(BMA)和N，N-二甲氨基丙基丙烯酰胺(DMAPAAm)的pH和温度响应性共聚物。用交联聚(NIPAAm-co-BMA-co-DMAPAAm)(IBD)水凝胶对氨基丙基硅胶小球进行了改性，并将其作为离子交换和温度响应层析的高效液相色谱填料。随着温度和pH的变化，固定相的表面性质由亲水变为疏水，由带电变为不带电。此外，由于温度的变化，离子交换基团可能会出现或隐藏在聚合物链表面。NSAIDs与该固定相的相互作用受温度和pH的控制，流动相恒定。PH和温度响应层析有望用于药物和生物分子的分离。(C)2006爱思唯尔B.V.保留所有权利。
We report on the development of a novel analytical HPLC technique of nonsteroidal anti-inflammatory drugs (NSAIDs), such as ibuprofen, ketoprofen and naproxen, with an isocratic aqueous mobile phase. In this study, we designed a new pH- and temperature-responsive copolymer of N-isopropylacrylamide (NIPAAm), butyl methacrylate (BMA) and N,N-dimethylaminopropylacrylamide (DMAPAAm). The copolymer was modified with cross-linked poly(NIPAAm-co-BMA-co-DMAPAAm) (IBD) hydrogel on to aminopropyl silica beads, and the products were evaluated as HPLC packing materials for an ion-exchange- and temperature-responsive chromatography. The property of the surface of the stationary phase was altered from hydrophilic to hydrophobic, and from charged to non-charged by changes in the temperature and pH. In addition, it is possible that ion-exchange groups can appear or be hidden on the polymer chain surface by temperature changes. The interactions of NSAIDs with this stationary phase were controlled by the temperature and the pH with a constant aqueous mobile phase. PH- and temperature-responsive chromatography is expected to be useful for the separation of pharmaceuticals and biomolecules. (c) 2006 Elsevier B.V. All rights reserved.