Physiological responses of Microcystis aeruginosa against the algicidal bacterium Pseudomonas aeruginosa
Physiological responses of Microcystis aeruginosa against the algicidal bacterium Pseudomonas aeruginosa
复制标题
铜绿微囊藻对杀藻菌铜绿假单胞菌的生理反应
DOI：
10.1016/j.ecoenv.2016.02.001
复制
发表时间：
2016
影响因子：
6.8
通讯作者：
Yin H
中科院分区：
文献类型：
--
作者：
Zhou Su;Yin Hua;Tang Shaoyu;Yin Donggao;Yang Yixuan;Liu Zehua;Dang Zhi;Peng Hui;Yin H
蓝藻在水生生态系统中的繁殖已经在世界范围内造成了水安全问题。我们的初步研究表明，铜绿假单胞菌对铜绿微囊藻的生长有抑制作用。以探讨其抑制OFP的作用机制。铜绿假单胞菌对细胞生长和胞内物质合成的影响。铜绿假单胞菌、叶绿素a、胞内蛋白质、碳水化合物、酶活性和离子代谢的浓度。铜绿假单胞菌，对其进行了调查。结果表明，OFP的杀藻效率为83.84%。治疗7d后铜绿假单胞菌消失。该菌株抑制了棉铃虫的繁殖。铜绿假单胞菌通过抑制蓝藻胞内蛋白质和碳水化合物的合成而受到抑制，暴露后仅检测到极少量的胞内蛋白质和碳水化合物。铜绿假单胞菌感染5d后，引起细胞内抗氧化酶活性的改变。铜绿假单胞菌，如过氧化氢酶、过氧化物酶。处理3d后，丙二醛的积累加重了膜的损伤。铜绿假单胞菌也影响了蓝藻的离子代谢。Na+和Cl-−的释放显著增加，K+、Ca~(2+)、Mg~(2+)、NO_3-−和SO_4~(2+)−的摄取减少。电子显微镜(SEM)和透射电子显微镜(TEM)分析表明，蓝藻和细菌细胞的表面形态和胞内结构随着时间的推移发生了巨大的变化。这些结果表明，OFP具有良好的杀藻活性。铜绿假单胞菌主要由发酵液OFP引起。铜绿假单胞菌阻碍细胞内蛋白质和碳水化合物的合成，通过膜脂过氧化损伤细胞膜。
Proliferation of cyanobacteria in aquatic ecosystems has caused water security problems throughout the world. Our preliminary study has showed that Pseudomonas aeruginosa can inhibit the growth of cyanobacterium, Microcystis aeruginosa. In order to explore the inhibitory mechanism ofP. aeruginosaon the cell growth and synthesis of intracellular substances ofM. aeruginosa, concentrations of Chlorophyll-a, intracellular protein, carbohydrate, enzyme activities and ion metabolism ofM. aeruginosa, were investigated. The results indicated that 83.84% algicidal efficiency ofP. aeruginosawas achieved after treatment for 7 days. The strain inhibited the reproduction ofM. aeruginosaby impeding the synthesis of intracellular protein and carbohydrate of cyanobacterium, and only a very small part of intracellular protein and carbohydrate was detected after exposure toP. aeruginosafor 5 days.P. aeruginosacaused the alteration of intracellular antioxidant enzyme activity ofM. aeruginosa, such as catalase, peroxidase. The accumulation of malondialdehyde aggravated membrane injury after treatment for 3 days.P. aeruginosaalso affected the ion metabolism of cyanobacteria. The release of Na+and Cl−was significantly enhanced while the uptake of K+, Ca2+, Mg2+, NO3−and SO42−decreased. Surface morphology and intracellular structure of cyanobacteria and bacterial cells changed dramatically over time as evidenced by electron microscope (SEM) and transmission electron microscope (TEM) analysis. These results revealed that the algicidal activity ofP. aeruginosawas primarily due to the fermentation liquid ofP. aeruginosathat impeded the synthesis of intracellular protein and carbohydrate, and damaged the cell membrane through membrane lipid peroxidation.