Starch granule size variation and relationship with tuber dry matter content in heritage potato varieties
Starch granule size variation and relationship with tuber dry matter content in heritage potato varieties
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DOI：
10.1016/j.scienta.2011.08.006
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发表时间：
2011-09
影响因子：
4.3
通讯作者：
Jichong Zhang;A. Murphy;Gong-she Liu;B. Bizimungu;Qiang Liu;Y. Leclerc;T. Xing;Xiuqing Li
中科院分区：
文献类型：
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作者：
Jichong Zhang;A. Murphy;Gong-she Liu;B. Bizimungu;Qiang Liu;Y. Leclerc;T. Xing;Xiuqing Li
加拿大马铃薯遗产品种是在加拿大植物品种登记制度正式建立之前从加拿大和世界各国收集的历史悠久的马铃薯品种，可能是马铃薯育种和生物制品开发中有益性状的宝贵基因资源。加强评价是加强使用这些材料的关键。目前还不清楚加拿大传统马铃薯品种之间淀粉粒的变化有多大。我们分析了淀粉颗粒大小的14个马铃薯遗产品种在加拿大植物基因资源收集超过2年，使用挤压果汁和显微镜的方法最近开发的马铃薯研究中心。品种表现出相当大的变化，淀粉颗粒的大小和形状。颗粒的平均长度从品种刚果的18μm到“Russet Burbank”的32μm不等。最大的单个颗粒，从“刚果”的64μm到“我们的克罗蒂”的91μm。不同品种间的粒度呈极显著正相关（r= 0.975，P <0.0001）。这种高重复性表明存在遗传因素决定淀粉颗粒大小。我们还发现，在这些遗产马铃薯中，淀粉颗粒大小与块茎干物质（以比重计）呈正相关。研究结果表明，这些马铃薯遗传品种的块茎淀粉粒大小和形状存在可重复的基因型变异，且与块茎干物质具有显著相关性，为通过选择育种进行淀粉粒遗传修饰提供了可能性和农艺相关性。
Heritage potato varieties in Canada are historic old varieties collected from Canada and various countries before the formal establishment of the Canadian plant variety registration system and may be a valuable gene resource for beneficial traits in potato breeding and bioproducts development. Greater evaluation is the key for the enhanced use of these materials. It is unknown how much variation of starch granules occurs among Canadian heritage potato varieties. We analyzed the starch granule size of 14 potato heritage varieties held in the Canadian Plant Gene Resources collection over 2 years using a squeezed juice and microscopic method recently developed at the Potato Research Centre. The varieties demonstrated considerable variation in starch granule size and shape. The granules showed average lengths ranging from 18μm in the variety Congo to 32μm in ‘Russet Burbank’. The largest single granule measured from 64μm in ‘Congo’ to 91μm in ‘Crotte d’Ours.’ The granule sizes of the varieties showed a very high correlation (r=0.975, P<0.0001) between years. This high reproducibility suggests the existence of genetic factors in determining starch granule size. We also found that the starch granule size is positively correlated with tuber dry matter (in terms of specific gravity) in these heritage potatoes. The results demonstrated reproducible genotypic variation for starch granule size and shape in tubers with a significant correlation to tuber dry matter in these heritage potato varieties, and offers the possibility for and agronomic relevance of genetic modification of starch granules through selective breeding.