N-Nitrosomorpholine Synthesis in Rodents Exposed to Nitrogen Dioxide and Morpholine
N-Nitrosomorpholine Synthesis in Rodents Exposed to Nitrogen Dioxide and Morpholine
复制标题
暴露于二氧化氮和吗啉的啮齿动物中 N-亚硝基吗啉的合成
DOI：
10.1021/bk-1981-0174.ch013
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
J. Sams
中科院分区：
文献类型：
--
作者：
S. Mirvish;P. Issenberg;J. Sams
本文研究了小鼠灌胃吗啉并暴露于大气中的NO_2时，体内产生N-亚硝基吗啉（NMOR）的可能性。使用一种pH值调节到1的“终止液”，其中含有抗坏血酸盐和氨基磺酸盐，以防止人为的亚硝化作用，并使用顺式-2，6-二甲基吗啉检测任何此类亚硝化作用，我们证明，在暴露于吗啉和NO2后，大鼠胃内容物或血液或整个小鼠中均未形成显著的NMOR。我们验证了Iqbalet等人（Science 207：1475，1980）的结果，即当用吗啉和NO2处理小鼠时产生NMOR，并且在不使用终止液的情况下处理含水甲醇中的整个小鼠匀浆。我们表明，NMOR是由亚硝化剂存在于NO2暴露小鼠的几个器官中的匀浆中产生的。
We examined the possibility thatN-nitrosomorpholine (NMOR) was producedin vivowhen mice were gavaged with morpholine and exposed to atmospheric nitrogen dioxide (NO2). Using a "stopping solution" adjusted to pH 1 that contained ascorbate and sulfamate to prevent artifactual nitrosation, andcis-2,6-dimethylmorpho1ine to detect any such nitrosation, we demonstrated that no significant NMOR was formed in rat stomach contents or blood, or the whole mouse, after exposure to morpholine and NO2. We verified the results of Iqbalet al. (Science207:1475, 1980) that NMOR was produced when mice were treated with morpholine and NO2, and the whole mouse homogenate in aqueous methanol was worked up without using a stopping solution. We showed that the NMOR was produced in the homogenate by a nitrosating agent present in several organs of NO2-exposed mice.