Effects of Graphene Oxide and Oxidized Carbon Nanotubes on the Cellular Division, Microstructure, Uptake, Oxidative Stress, and Metabolic Profiles
Effects of Graphene Oxide and Oxidized Carbon Nanotubes on the Cellular Division, Microstructure, Uptake, Oxidative Stress, and Metabolic Profiles
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氧化石墨烯和氧化碳纳米管对细胞分裂、微观结构、摄取、氧化应激和代谢特征的影响
DOI：
10.1021/acs.est.5b02102
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发表时间：
2015-09-15
影响因子：
11.4
通讯作者：
Zhou, Qaing
中科院分区：
文献类型：
--
作者：
Hu, Xiangang;Ouyang, Shaohu;Zhou, Qaing
纳米材料氧化物是自然环境中纳米材料的常见形态。本文比较了典型的氧化石墨烯(GO)和羧基单壁碳纳米管(C-SWCNT)的纳米毒理学。结果表明，在纳米材料作用后24 h，普通小球藻的细胞分裂受到促进，96 h后细胞分裂受到抑制。96h时，GO和C-SWCNT对细胞分裂的抑制率分别为0.08~15%和0.8~28.3%。GO和C-SWCNT均覆盖于细胞表面，但C-SWCNT的摄取百分率是GO的2倍。C-SWCNT较GO引起更强的质膜溶解和线粒体膜电位损失，细胞存活率下降更明显。此外，C-SWCNT暴露的细胞比GO暴露的细胞表现出更多的淀粉粒和溶酶体形成，以及更高的活性氧(ROS)水平。代谢组学分析显示，对照组、C-SWCNT组和GO组之间的代谢谱有显著差异。烷烃、赖氨酸、十八碳二烯酸和缬氨酸的代谢与ROS有关，可作为ROS的新生物标志物。纳米毒理学机制包括抑制脂肪酸、氨基酸和小分子酸的代谢。这些发现为GO和C-SWCNT对细胞反应的影响提供了新的见解。
Nanomaterial oxides are common formations of nanomaterials in the natural environment. Herein, the nanotoxicology of typical graphene oxide (GO) and carboxyl single-walled carbon nanotubes (C-SWCNT) was compared. The results showed that cell division of Chlorella vulgaris was promoted at 24 h and then inhibited at 96 h after nanomaterial exposure. At 96 h, GO and C-SWCNT inhibited the rates of cell division by 0.08-15% and 0.8-28.3%, respectively. Both GO and C-SWCNT covered the cell surface, but the uptake percentage of C-SWCNT was 2-fold higher than that of GO. C-SWCNT induced stronger plasmolysis and mitochondrial membrane potential loss and decreased the cell viability to a greater extent than GO. Moreover, C-SWCNT-exposed cells exhibited more starch grains and lysosome formation and higher reactive oxygen species (ROS) levels than GO-exposed cells. Metabolomics analysis revealed significant differences in the metabolic profiles among the control, C-SWCNT and GO groups. The metabolisms of alkanes, lysine, octadecadienoic acid and valine was associated with ROS and could be considered as new biomarkers of ROS. The nanotoxicological mechanisms involved the inhibition of fatty acid, amino acid and small molecule acid metabolisms. These findings provide new insights into the effects of GO and C-SWCNT on cellular responses.