Ontogeny of type 1 angiotensin II receptor gene expression in the rat.
Ontogeny of type 1 angiotensin II receptor gene expression in the rat.
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大鼠 1 型血管紧张素 II 受体基因表达的个体发育。
DOI：
10.1172/jci116232
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Gomez,RA
中科院分区：
文献类型：
--
作者：
Tufro-McReddie,A;Harrison,JK;Everett,AD;Gomez,RA
为了确定1型血管紧张素II受体（AT 1）基因的表达是否受发育调控以及这种调控是否具有组织特异性，使用1133 bp大鼠AT 1 cDNA，通过北方印迹分析测定了胎儿、新生和成年大鼠肝脏和肾脏中AT 1 mRNA水平。在肝脏中，AT 1 mRNA水平从妊娠15天到5天龄增加了5倍。5日龄大鼠肝脏AT 1 mRNA水平与成年大鼠相似。在肾脏中，AT 1 mRNA水平在未成熟动物中高于成年动物。原位杂交法检测AT 1 mRNA在大鼠肾内的分布。在成人中，AT 1 mRNA存在于肾小球，动脉，和直小血管，而在新生儿AT 1 mRNA也观察到在肾皮质区。我们的结论是：（a）胎儿肾脏和肝脏表达AT 1基因;（B）AT 1基因的表达以组织特异性的方式受到发育调节;（c）在成熟过程中，肾脏中AT 1 mRNA的定位从广泛分布在生肾皮质转移到肾小球、动脉和直血管的特定位点，表明血管紧张素受体在肾单位生长和发育中的作用。图片
To determine whether the expression of the type 1 angiotensin II receptor (AT1) gene is developmentally regulated and whether the regulation is tissue specific, AT1 mRNA levels were determined by Northern blot analysis in livers and kidneys from fetal, newborn, and adult rats, using a 1133-bp rat AT1 cDNA. In the liver, AT1 mRNA levels increased fivefold from 15 d gestation to 5 d of age. Liver AT1 mRNA levels at 5 d of age were similar to those of adult rats. In the kidney, AT1 mRNA levels were higher in immature than in adult animals. The intrarenal distribution of AT1 mRNA was assessed by in situ hybridization to a 35S-labeled 24 residues oligonucleotide complementary to rat AT1 mRNA. In the adult, AT1 mRNA was present in glomeruli, arteries, and vasa recta, whereas in the newborn AT1 mRNA was observed also over the nephrogenic area of the cortex. We conclude that: (a) fetal kidney and liver express the AT1 gene; (b) the AT1 gene expression is developmentally regulated in a tissue-specific manner; (c) during maturation, localization of AT1 mRNA in the kidney shifts from a widespread distribution in the nephrogenic cortex to specific sites in glomeruli, arteries, and vasa recta, suggesting a role for the angiotensin receptor in nephron growth and development.Images