HIF-1alpha may promote glycolysis in psoriasis vulgaris via upregulation of CD147 and GLUT1.
HIF-1alpha may promote glycolysis in psoriasis vulgaris via upregulation of CD147 and GLUT1.
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DOI：
10.11817/j.issn.1672-7347.2021.200010
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发表时间：
2021-04-28
期刊：
影响因子：
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通讯作者：
Su, Juan
中科院分区：
文献类型：
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作者：
Tang, Wen;Long, Tingting;Su, Juan
目的：分析缺氧诱导因子-1 α（hypoxia inducible factor-1 alpha，HIF-1 α）在大鼠心肌中的表达及分布本研究旨在检测寻常型银屑病患者和正常人表皮中HIF-1 α、CD 147和葡萄糖转运蛋白1（GLUT 1）的表达，探讨这些蛋白之间的相互关系及其在缺氧HaCaT细胞系中的作用。采用免疫组化法检测48例寻常型银屑病患者和33例健康人皮肤组织中HIF-1 α、CD 147和GLUT 1的表达水平。将氯化钴（CoCl 2）加入HaCaT细胞的培养基中以模拟缺氧，同时使用RNA干扰和转染技术通过定量实时聚合酶链反应和Western印迹来探索这些蛋白之间的关联。结果：HIF-1 α、CD 147、GLUT 1在寻常型银屑病皮损中高表达，糖酵解能力增强; HIF-1 α上调CoCl 2处理的HaCaT细胞中CD 147和GLUT 1的表达，增加乳酸产生并降低ATP水平，结论：寻常型银屑病患者角质形成细胞损伤后糖酵解能力增强，提示HIF-1 α、CD 147和GLUT 1与糖酵解有关，有望成为银屑病治疗的靶点。
OBJECTIVES: To analyze the expressions and distributions of hypoxia-inducible factor-1alpha (HIF-1alpha), CD147, and glucose transporter 1 (GLUT1) in epidermis from psoriasis vulgaris and normal people, and to explore the associations among these proteins and their roles in hypoxic HaCaT cell line.METHODS: The expression levels of HIF-1alpha, CD147, and GLUT1 were determined by immunohistochemistry staining in skin biopsies from 48 psoriasis vularis patients and 33 healthy subjects. Cobalt chloride (CoCl2) was added into the culture media of HaCaT cells to mimic hypoxia while RNA interference and transfection technologies were used to explore the association among these proteins by quantitative real-time polymerase chain reaction and Western blotting. Glycolytic capacity was detected by ATP and lactate measurements.RESULTS: HIF-1alpha, CD147, and GLUT1 were highly expressed and the glycolytic capacity was increased in lesions of psoriasis vulgaris; HIF-1alpha upregulated the expression of CD147 and GLUT1, increased the lactate production and decreased the ATP level in CoCl2-treated HaCaT cells, while CD147 and GLUT1 directly or indirectly bound to each other.CONCLUSIONS: Glycolytic capacity increases in the injured keratinocytes of psoriasis vulgaris, suggesting that HIF-1alpha, CD147, and GLUT1 are associated with glycolysis, which can be considered as the promising targets for psoriasis therapy.