Ascherxanthone B from Aschersonia luteola, a new antifungal compound active against rice blast pathogen Magnaporthe grisea
Ascherxanthone B from Aschersonia luteola, a new antifungal compound active against rice blast pathogen Magnaporthe grisea
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DOI：
10.1111/j.1365-2672.2009.04349.x
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发表时间：
2009-11-01
影响因子：
4
通讯作者：
Kirtikara, K.
中科院分区：
文献类型：
--
作者：
Chutrakul, C.;Boonruangprapa, T.;Kirtikara, K.
目的：稻瘟病菌（Magnaporthe grisea）对杀菌剂存在抗药性，需要新的活性剂。方法和结果：一个新的，可靠的体外筛选系统的基础上建立了一个微量稀释平板格式使用的M. grisea THL 16.研究了来自大约800种真菌菌株的培养液提取物，发现其中一种，Aschersonia luteola BCC 8774，产生活性杀真菌化合物ascherxanthone B，其IC 90值为0中心点58 μ g ml-1（0中心点95 μ mol l-1）。一项关于ascherxanthone B抗稻瘟病效力的体内研究表明，在减少疾病方面具有积极作用。对该种的研究表明，A. luteola BCC 8774，导致发现了相关的新代谢产物，ascherxanthone B具有杀菌特性。研究的意义和影响：目前的稻瘟病控制方法似乎失败，导致作物损失增加。我们发现的抗稻瘟病活性所显示的ascherxanthone B是第一步，在一个潜在的新型杀菌剂的发展。
Aims:Fungicide resistance now exists in the rice blast fungus, Magnaporthe grisea, necessitating the need for new active agents. Fungi isolated from habitats in Thailand were screened with reference to this problem.Methods and Results:A new, reliable in vitro screening system based on a microdilution plate format was set up using a virulent strain of M. grisea THL 16. Culture broth extracts from approximately 800 fungal strains were investigated, one of these, Aschersonia luteola BCC 8774, was found to produce an active fungicidal compound, ascherxanthone B, with an IC90 value of 0 center dot 58 mu g ml-1 (0 center dot 95 mu mol l-1). An in vivo study of anti-blast efficacy of ascherxanthone B showed a positive effect in disease reduction.Conclusions:Previous report has shown that a species of Aschersonia produces ascherxanthone A. Research on the species, A. luteola BCC 8774, led to the discovery of related novel metabolite, ascherxanthone B with fungicidal properties.Significance and Impact of the Study:Current methods of rice blast control seem to fail leading to increase in crop losses. Our discovery of the anti-blast activity shown by ascherxanthone B is the first step in the development of a potentially novel fungicide.