Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice
Canalostomy As a Surgical Approach to Local Drug Delivery into the Inner Ears of Adult and Neonatal Mice
复制标题
耳管造口术作为一种将局部药物输送到成年和新生小鼠内耳的手术方法
DOI：
10.3791/57351
复制
发表时间：
2018-05-01
影响因子：
1.2
通讯作者：
Gong, Shu-Sheng
中科院分区：
文献类型：
--
作者：
Guo, Jing-Ying;He, Lu;Gong, Shu-Sheng
内耳局部给药是治疗内耳疾病的一种很有前途的疗法。经半规管注射(导管造口术)已被证明是一种将局部药物输送到内耳的有效方法。这篇文章的目的是详细描述成年和新生小鼠导管造口术中涉及的外科技术。绿色染料和携带绿色荧光蛋白基因的腺相关病毒血清8型表明，导管造口术促进了注射试剂在耳蜗和前庭终器中的广泛分布，对听力和前庭功能的损害最小。该手术成功地在成年和新生小鼠身上实施；事实上，如果需要的话，可以进行多次手术。综上所述，导管造口术是一种安全有效的药物进入成年和新生小鼠内耳的方法，将来可能用于治疗人类内耳疾病。
Local delivery of therapeutic drugs into the inner ear is a promising therapy for inner ear diseases. Injection through semicircular canals (canalostomy) has been shown to be a useful approach to local drug delivery into the inner ear. The goal of this article is to describe, in detail, the surgical techniques involved in canalostomy in both adult and neonatal mice. As indicated by fast-green dye and adeno-associated virus serotype 8 with the green fluorescent protein gene, the canalostomy facilitated broad distribution of injected reagents in the cochlea and vestibular end-organs with minimal damage to hearing and vestibular function. The surgery was successfully implemented in both adult and neonatal mice; indeed, multiple surgeries could be performed if required. In conclusion, canalostomy is an effective and safe approach to drug delivery into the inner ears of adult and neonatal mice and may be used to treat human inner ear diseases in the future.