Corn gluten hydrolysate regulates the expressions of antioxidant defense and ROS metabolism relevant genes in H2O2-induced HepG2 cells
Corn gluten hydrolysate regulates the expressions of antioxidant defense and ROS metabolism relevant genes in H2O2-induced HepG2 cells
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玉米麸质水解物调节H2O2诱导的HepG2细胞抗氧化防御和ROS代谢相关基因的表达
DOI：
10.1016/j.jff.2017.12.056
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发表时间：
2018-03-01
影响因子：
5.6
通讯作者：
Liu, Jingbo
中科院分区：
文献类型：
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作者：
Wang, Liying;Ding, Long;Liu, Jingbo
活性氧（ROS）和抗氧化剂可以影响许多基因的表达。本研究旨在探讨玉米谷蛋白水解物（CGH）对HepG2细胞抗氧化防御及ROS代谢相关基因表达的影响。共分析了84个参与抗氧化防御和ROS代谢的基因。结果显示，GPX3、GPX5、SOD3、CYGB、SEPP1、MT3等基因在氧化损伤的HepG2细胞中下调，而在CGH1（分子量< 1 kDa）预处理的细胞中上调，说明CGH1可以改善最初被氧化应激抑制的几种抗氧化基因的表达。此外，通路分析表明CGH1的抗氧化能力与花生四烯酸代谢相关（P < 0.05）。CGH1降低EPHX2的表达，可能通过增加细胞环氧二碳三烯酸（EET）和形成EET磷脂来保护细胞膜免受h2o2诱导的细胞膜损伤。
Reactive oxygen species (ROS) and antioxidants are known to influence the expressions of a number of genes. This study aimed to investigate the effects of corn gluten hydrolysate (CGH) on the expressions of antioxidant defense and ROS metabolism relevant genes in HepG2 cells. Eighty-four genes involving in antioxidant defense and ROS metabolism were analyzed. The results showed that the genes such as GPX3, GPX5, SOD3, CYGB, SEPP1 and MT3 that down-regulated in oxidative damaged HepG2 cells were, however, up-regulated in CGH1 (molecular weights < 1 kDa)-pretreated cells, suggesting that CGH1 could improve the expressions of several antioxidant genes that were initially suppressed by oxidative stress. In addition, pathway analysis demonstrated that antioxidant ability of CGH1 was associated with arachidonic acid metabolism (P < .05). CGH1 reduced the expression of EPHX2, which may protect against H2O2-induced cell membrane damage by increasing cellular epoxyeicosatrienoic acid (EET) and forming EET-phospholipids for cell membrane.