The characterization of Bordetella pertussis strains isolated in the Central-Western region of Brazil suggests the selection of a specific genetic profile during 2012-2014 outbreaks
The characterization of Bordetella pertussis strains isolated in the Central-Western region of Brazil suggests the selection of a specific genetic profile during 2012-2014 outbreaks
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DOI：
10.1017/s0950268816003332
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发表时间：
2017-05-01
影响因子：
4.2
通讯作者：
Campos, T. A.
中科院分区：
文献类型：
--
作者：
Rocha, E. L.;Leite, D.;Campos, T. A.
百日咳是一种由百日咳杆菌引起的全球性急性呼吸道疾病。尽管疫苗覆盖率很高，但这种细菌继续在人群中传播，仍然是最常见的疫苗可预防疾病之一。在巴西，自1990年以来百日咳发病率显著下降，但自2011年以来观察到发病率突然上升。因此，本研究的目的是对2012年8月至2014年8月在巴西中西部地区（特别是联邦区）分离的百日咳菌株进行分子流行病学特征分析。在此期间，从暴发中分离出92株百日咳菌株。所有菌株均采用血清分型和XbaI脉冲场凝胶电泳图谱进行鉴定。2012年8 - 12月以1,3型（13/17）最为流行。2013年1、3型患病率下降（13/30），2014年1 - 8月以1、2型患病率最高（33/45）。确定了14个PFGE剖面。其中BP-XbaI0039患病率从2012年的3/17上升到2013年的10/30,2014年的35/45。这些结果证明了在这一时期特定遗传谱的选择，表明具有高循环潜力的细菌基因组谱的发生。
Pertussis is a worldwide acute respiratory disease caused by the bacterium Bordetella pertussis. Despite high vaccine coverage, the bacterium continues to circulate in populations and is still one of the most common vaccine-preventable diseases. In Brazil, pertussis incidence has presented a significant decrease since 1990 but since 2011 a sudden increase in incidence has been observed. Thus, the aim of this study was to perform a molecular epidemiological characterization of B. pertussis strains isolated in the Central-Western region (specifically in Distrito Federal) of Brazil from August 2012 to August 2014. During this period, 92 B. pertussis strains were isolated from the outbreaks. All strains were characterized by serotyping and XbaI pulsed-field gel electrophoresis profiles. From August to December 2012, the most prevalent serotype observed was 1,3 (13/17). During 2013 the prevalence of serotype 1,3 decreased (13/30) and from January 2014 to August 2014 the most prevalent serotype was 1,2 (33/45). Fourteen PFGE profiles were identified. Of these, BP-XbaI0039 prevalence increased from 3/17 in 2012 to 10/30 in 2013, and 35/45 in 2014. These results evidence the selection of a specific genetic profile during this period, suggesting the occurrence of a bacterial genomic profile with high circulation potential.