New viral biogeochemical roles revealed through metagenomic analysis of Lake Baikal.
New viral biogeochemical roles revealed through metagenomic analysis of Lake Baikal.
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DOI：
10.1186/s40168-020-00936-4
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发表时间：
2020-11-19
期刊：
影响因子：
15.5
通讯作者：
Rodriguez-Valera F
中科院分区：
文献类型：
--
作者：
Coutinho FH;Cabello-Yeves PJ;Gonzalez-Serrano R;Rosselli R;López-Pérez M;Zemskaya TI;Zakharenko AS;Ivanov VG;Rodriguez-Valera F
贝加尔湖是地球上最大的液态淡水水体。先前的研究已经描述了该栖息地的微生物组成，但该生态系统的病毒群落尚未详细描述。在这里，我们描述了该栖息地在深度和季节梯度上的病毒多样性。我们发现了 19,475 个真实的病毒序列，这些序列源自预计会感染贝加尔湖中丰富且具有重要生态意义的类群的病毒，例如硝化螺菌门、甲基嗜菌科和泉生菌门。多样性分析揭示了上层和深海区域之间病毒群落组成的显着变化。对单个病毒群体的基因内容的分析使我们能够描述感染 Nitrospirota 的第一批噬菌体之一，以及它们广泛的辅助代谢基因库，这些基因可能通过还原 TCA 循环增强碳固定。我们还描述了具有增强甲醇氧化和 S-腺苷-L-蛋氨酸循环潜力的甲基营养细菌的噬菌体。这些发现揭示了病毒影响淡水生态系统碳循环的新方式，即通过使用作用于暗碳固定和甲基营养代谢的辅助代谢基因。因此，我们的结果揭示了病毒影响具有重大生态相关性的生物地球化学循环的过程。视频摘要 在线版本包含可在 10.1186/s40168-020-00936-4 获取的补充材料。
Lake Baikal is the largest body of liquid freshwater on Earth. Previous studies have described the microbial composition of this habitat, but the viral communities from this ecosystem have not been characterized in detail. Here, we describe the viral diversity of this habitat across depth and seasonal gradients. We discovered 19,475 bona fide viral sequences, which are derived from viruses predicted to infect abundant and ecologically important taxa that reside in Lake Baikal, such as Nitrospirota, Methylophilaceae, and Crenarchaeota. Diversity analysis revealed significant changes in viral community composition between epipelagic and bathypelagic zones. Analysis of the gene content of individual viral populations allowed us to describe one of the first bacteriophages that infect Nitrospirota, and their extensive repertoire of auxiliary metabolic genes that might enhance carbon fixation through the reductive TCA cycle. We also described bacteriophages of methylotrophic bacteria with the potential to enhance methanol oxidation and the S-adenosyl-L-methionine cycle. These findings unraveled new ways by which viruses influence the carbon cycle in freshwater ecosystems, namely, by using auxiliary metabolic genes that act upon metabolisms of dark carbon fixation and methylotrophy. Therefore, our results shed light on the processes through which viruses can impact biogeochemical cycles of major ecological relevance. Video Abstract The online version contains supplementary material available at 10.1186/s40168-020-00936-4.
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影响因子：
14.9
作者：
Finn RD;Clements J;Arndt W;Miller BL;Wheeler TJ;Schreiber F;Bateman A;Eddy SR
通讯作者：
Eddy SR
影响因子：
64.8
作者：
Danovaro, Roberto;Dell'Anno, Antonio;Weinbauer, Markus
通讯作者：
Weinbauer, Markus
影响因子：
11.3
作者：
Edwards RA;McNair K;Faust K;Raes J;Dutilh BE
通讯作者：
Dutilh BE
影响因子：
16.6
作者：
Coutinho FH;Silveira CB;Gregoracci GB;Thompson CC;Edwards RA;Brussaard CPD;Dutilh BE;Thompson FL
通讯作者：
Thompson FL
影响因子：
4.5
作者：
Cabello-Yeves, Pedro J.;Zemskaya, Tamara, I;Rodriguez-Valera, Francisco
通讯作者：
Rodriguez-Valera, Francisco