Differential gene expression in planktonic and biofilm cells of multiple antibiotic-resistant Salmonella Typhimurium and Staphylococcus aureus
Differential gene expression in planktonic and biofilm cells of multiple antibiotic-resistant Salmonella Typhimurium and Staphylococcus aureus
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DOI：
10.1111/j.1574-6968.2011.02429.x
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发表时间：
2011-12-01
影响因子：
2.1
通讯作者：
Ahn, Juhee
中科院分区：
文献类型：
--
作者：
He, Xinlong;Ahn, Juhee
本研究旨在评估调节至pH 5.5和pH 7.3的胰蛋白胨大豆肉汤中金黄色葡萄球菌和鼠伤寒沙门氏菌的增殖和生物膜细胞的基因表达模式。多重耐药链球菌的增殖细胞和生物膜细胞。金黄色葡萄球菌（S. aureusR）和S.鼠伤寒沙门氏菌（S. TyphimuriumR）对β-内酰胺类抗生素的耐药性高于敏感菌株。金黄色葡萄球菌（S. aureusS）和S.鼠伤寒沙门氏菌（S. TyphimuriumS）在pH 5.5和pH 7.3下。norB、norC和mdeA基因的相对基因表达水平在S. norB、norC、mdeA、sec、seg、sei、sel、sem、sen和seo基因在金黄色葡萄球菌的生物膜细胞中是稳定的。金色河本研究为了解耐药病原菌在酸性胁迫下的代谢和生物膜细胞中的基因表达模式提供了有用的信息。
This study was designed to evaluate gene expression patterns of the planktonic and biofilm cells of Staphylococcus aureus and Salmonella Typhimurium in trypticase soy broth adjusted to pH 5.5 and pH 7.3. The planktonic and biofilm cells of multiple antibiotic-resistant S. aureus (S. aureusR) and S. Typhimurium (S. TyphimuriumR) were more resistant to beta-lactams than those of antibiotic-susceptible S. aureus (S. aureusS) and S. Typhimurium (S. TyphimuriumS) at pH 5.5 and pH 7.3. The relative gene expression levels of norB, norC, and mdeA genes were increased by 7.0-, 4.7-, and 4.6-fold, respectively, in the biofilm cells of S. aureusS grown at pH 7.3, while norB, norC, mdeA, sec, seg, sei, sel, sem, sen, and seo genes were stable in the biofilm cells of S. aureusR. This study provides useful information for understanding gene expression patterns in the planktonic and biofilm cells of antibiotic-resistance pathogens exposed to acidic stress.