Gene profiling of the red light signalling pathways in roots
Gene profiling of the red light signalling pathways in roots
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DOI：
10.1093/jxb/erl086
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发表时间：
2006-09-01
影响因子：
6.9
通讯作者：
Correll, Melanie J.
中科院分区：
文献类型：
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作者：
Molas, Maria Lia;Kiss, John Z.;Correll, Melanie J.
红光通过光敏色素起作用，控制着植物发育的许多方面。许多下游的光敏色素的信号转导元件已被确定在植物的地上部分，然而，很少有元素的红光信号已被确定为具体的根。利用基因芯片和实时定量PCR技术对拟南芥幼苗在红光照射1 h后根中的基因表达变化进行了研究。几个因素的下游光敏色素的红光信号在根中进行了鉴定。在这项研究中发现的一些差异表达的基因已经在整个植物的红光信号通路中得到了表征。例如，植物色素激酶1（PKS 1），长下胚轴5（HY 5），早开花4（ELF 4），和巨根茶（GI）都显着上调暴露于1小时的红光的幼苗的根。植物色素A反应的供应者1（SPA 1）和组成型光形态发生1样（COP 1-like）基因的上调表明，PHYA介导的途径被红光减弱。此外，基因参与侧根和根毛的形成，根质体发育，苯丙烷代谢，激素信号也受到红光照射。有趣的是，RPT 2/NPH 3（ROOT PHOTOTROPIC 2/NON PHOTOTROPIC HYPOCOTYL 3）家族的成员，已被证明介导蓝光诱导的向光性，在红光下的根中也受到差异调节。因此，这些结果表明，红光和蓝光的途径相互作用的幼苗的根，并在植物的地上部分中发现的红光信号中涉及的许多元素的差异表达在根内1小时的红光曝光。
Red light, acting through the phytochromes, controls numerous aspects of plant development. Many of the signal transduction elements downstream of the phytochromes have been identified in the aerial portions of the plant; however, very few elements in red-light signalling have been identified specifically for roots. Gene profiling studies using microarrays and quantitative Real-Time PCR were performed to characterize gene expression changes in roots of Arabidopsis seedlings exposed to 1 h of red light. Several factors acting downstream of phytochromes in red-light signalling in roots were identified. Some of the genes found to be differentially expressed in this study have already been characterized in the red-light-signalling pathway for whole plants. For example, PHYTOCHROME KINASE 1 (PKS1), LONG HYPOCOTYL 5 (HY5), EARLY FLOWERING 4 (ELF4), and GIGANTEA (GI) were all significantly up-regulated in roots of seedlings exposed to 1 h of red light. The up-regulation of SUPPRESSOR OF PHYTOCHROME A RESPONSES 1 (SPA1) and CONSTITUTIVE PHOTOMORPHOGENIC 1-like (COP1-like) genes suggests that the PHYA-mediated pathway was attenuated by red light. In addition, genes involved in lateral root and root hair formation, root plastid development, phenylpropanoid metabolism, and hormone signalling were also regulated by exposure to red light. Interestingly, members of the RPT2/NPH3 (ROOT PHOTOTROPIC 2/NON PHOTOTROPIC HYPOCOTYL 3) family, which have been shown to mediate blue-light-induced phototropism, were also differentially regulated in roots in red light. Therefore, these results suggest that red and blue light pathways interact in roots of seedlings and that many elements involved in red-light-signalling found in the aerial portions of the plant are differentially expressed in roots within 1 h of red light exposure.